中国寄生虫学与寄生虫病杂志 ›› 1990, Vol. 8 ›› Issue (3): 207-209.

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Dot-ELISA在脑囊虫病诊断中的应用

姜洪杰,杨连雪,庞苏红,孙铁,谢淑萍,孟家眉,温桂芝,王国志   

  1. 首都医学院寄生虫学教研室; 首都医学院寄生虫学教研室; 首都医学院寄生虫学教研室; 首都医学院寄生虫学教研室; 北京宣武医院脑囊虫病研究室; 北京宣武医院脑囊虫病研究室; 佳木斯医学院寄生虫学教研室; 佳木斯医学院寄生虫学教研室
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1990-08-31 发布日期:2017-01-09

DOT-ELISA IN THE DIAGNOSIS OF NEUROCYSTICERCOSIS

  • Received:2017-01-09 Revised:2017-01-09 Online:1990-08-31 Published:2017-01-09

摘要: 本文报告用Dot-ELISA法检测脑囊虫病患者的血清和脑脊液的抗体。108例确诊为脑囊虫病患者的血清阳性率为81.6~96.1%,14例确诊为脑囊虫病患者脑脊液的阳性率为85.7~100%。54例正常人血清在1:40稀释度以上,均未出现假阳性;与并殖吸虫病患者及华支睾吸虫病患者血清均未出现交叉反应;与包虫病及脑血管疾病患者血清有一定的交叉反应。

关键词: Dot-ELISA, 脑囊虫病, 诊断

Abstract: The doteRzyme-linked immunosorbent assa was used to detect Cysticercus cellulosae antibodies in sera of patients with neurocysticercosis. Among 108 confirmed cases of neurocysticercosis 81.6-96.1% showed positive reactions. Two out of 54 normal control sera reacted at a serum dilution of 1:20, but none at a 1:40 (range 40-640). No cross reactions were observed with sera from cases of paragonimiasis and clonorchiasis, but it did occur to some extent with sera from cases of echinococcosis and cerebrovascular diseases. The results indicated that the dot-ELISA was sensitive, specific and economic for the diagnosis of neurocysticercosis.