中国寄生虫学与寄生虫病杂志 ›› 1989, Vol. 7 ›› Issue (4): 276-279.
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郑惠君,陶增厚,张晓辉,Willy F.Piessens
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摘要: 在4种培养系统中,马来丝虫Ⅲ期幼虫在改良RPMI1640、20%小牛血清和人胚肾细胞系为饲养层的培养系统中生长发育良好,可以从Ⅲ期幼虫蜕皮2次发育到童虫,最长存活时间为54d。在单纯改良RPMI1640、TC199和20%小牛血清中培养,从Ⅲ期幼虫发育到童虫的最长存活时间为42d。班氏丝虫Ⅲ期幼虫在上述2种培养系统中,分别存活57和36d,从Ⅲ期幼虫蜕皮进入Ⅳ期幼虫和童虫。对马来丝虫幼虫体外培养蜕皮时间、虫体大小与沙鼠体内发育情况进行了比较。
关键词: 班氏丝虫, 马来丝虫, 感染期幼虫, 体外培养, 童虫
Abstract: This paper reported on improved in vitro cultute system of human lymphatic filarial larvae. Four culture systems were used. Third-stage larvae of Brugia malayi were best maintained, developing and molted twice in the medium containing modified RPMI-1640 medium, supplemented with 20% newborn calf serum and fauman embrsyonic kidney cell line as feeder layer. This culture system kept larvae alive up to 54 days. Brugia malayl third-stage larvae began to moult on the 8-10th day and again on the 32-36th day; Wuchereria bancrofti third stage larvae grew and developed to the fourth-stage and juvenile and survived to 57 days. They began to moult on the 12-18th days and again on the 32-44th day. This culture system was thought to be useful for studies on morphology and sensitivity to drugs.We also studied several cell-free culture systems. Among them, Best survival, growth and devclopment were obtained in 1:1 mixture of modified RPMI-1640 and TC199 medium supplemented with 20% newborn calf serum. Both Wuchereria bancrofti. and Brugia malayi third-stage larvae grew and developed to the fourth stage larvae and juveniles and survived to 36 days and 42 days respectively. The availability of such culture systems for human filariasis would facilitate studies of biochemistry, immunology, duction of moncclonal antibodies and vaccine.
郑惠君,陶增厚,张晓辉,Willy F.Piessens. 班氏丝虫和马来丝虫感染期幼虫体外培养的进一步研究[J]. 中国寄生虫学与寄生虫病杂志, 1989, 7(4): 276-279.
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https://www.jsczz.cn/CN/Y1989/V7/I4/276