中国寄生虫学与寄生虫病杂志 ›› 1989, Vol. 7 ›› Issue (1): 53-56.

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离体培养约氏疟原虫动合子形成的扫描电镜观察

高兴政   

  1. 北京医科大学寄生虫学教研室
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1989-02-28 发布日期:2017-01-09

SCANNING ELECTRON MICROSCOPIC OBSERVATIONS OF OOKINETE FORMATION OF PLASMODIUM YOELII YOELII IN VITRO

  • Received:2017-01-09 Revised:2017-01-09 Online:1989-02-28 Published:2017-01-09

摘要: 本文用扫描电镜观察约氏疟原虫动合子形成的过程。首先从合子一端伸出指状或棒状突起,突起顶端呈平截状圆锥形,随着突起伸长、变粗,合子本体逐渐皱缩变小,最终形成残余体。在新形成的动合子和合子本体的残余体之间增厚,并由此断离。在动合子形成期间虫体运动活跃.出现向前伸长和扭曲运动。成熟动合子表面光滑,呈香蕉形或腊肠形.虫体前端为顶端复合体,在前端截状圆锥体后因弯曲、收缩活动而出现多个表膜皱折。动合子本身有扭曲运动,因而虫体表面出现螺旋状皱纹。

关键词: 动合子, 约氏疟原虫, 扫描电镜观察, 离体培养, 残余体, 本体, 圆锥体, 光学显微镜, 指状突起, 固定标本

Abstract: The ookinete formation and mature ookinete of Plasmodium yoelii yoelii were described. During the development from zygote to ookinete, at first a finger-shaped or a stick-shaped projection protruded from one side of zygote (Fig. l).The anterior end of the projection assumed a truncated cone shape (Figs. 1-5). Following enlargement of the projection, the body of zygote gradually wrinkled and shrank and became smaller (Figs. 2-5). An annular structure developed between the newly formed ookinete and the remaining body of zygote (Figs. 5-6), which ultimately separated from the ookinete.Tile ookinete was banana-shaped. The surface of mature ookinetes was smooth (Fig. 13). On the tip there was an apical complex showing truncated cone shape. Behind the apical end there were a few pellicular folds (Figs. 9-11).Through the course of development of the ookinete the parasite constantly showed obvious movement. As the parasites protruding forward, longitudinal and spiral wrinkles appeared on their body surface (Figs. 2-4, 10-12).