关键词: 对流免疫电泳法, 按蚊胃血标本, 醋酸纤维膜, 环状沉淀试验, 醋纤膜, 中华按蚊, 抗血清, 寄生虫病研究所, 同时对比, 稀释度

Abstract: A counter immunoelectrophoresis test using cellulose acetate membrane(CAMCIEP) was developed to identify the host source of mosquito blood meals. The optimum dilutions of antiserum and antigen for the test are 1:8 and 1:500 respectively. The technique was used on blood meal specimens from Anopheles sinensis 4, 12 and 24 hours after feeding through a membrane on fresh human or pig blood and after 6, 16 and 29 months storage at 4℃.1557 specimens collected from the field were examined and compared with the precipitin ring test (PRT). The results of the two methods were in good agreement. Similar results were also found in the identification of 444 specimens collected from the field using CAMCIEP and agar gel counter immunoelectrophoresis (AGCIEP), the positive rates were 99.8% in CAMCIEP and 99.3% in AGCIEP. It is suggested that the CAMCIEP test could be used to identify the host source of mosquito blood meals.