中国寄生虫学与寄生虫病杂志 ›› 1985, Vol. 3 ›› Issue (3): 198-200.

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纸片放射抗原吸附试验诊断肺吸虫病的初步研究

王恩荣,段云芬,刘玉珍,宋昌存,寿干城,汪小卫,李得垣   

  1. 中国医科大学寄生虫学教研室; 浙江人民卫生实验院寄生虫病研究所; 中国医科大学寄生虫学教研室; 浙江人民卫生实验院寄生虫病研究所; 浙江人民卫生实验院寄生虫病研究所; 浙江人民卫生实验院寄生虫病研究所; 中国医科大学寄生虫学教研室
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1985-08-31 发布日期:2017-01-12

PRELIMINARY STUDIES ON PAPER RADIOANTIGENSOHBENT TEST IN THE DIAGNOSIS OF PARAGONIMIASIS

  • Received:2017-01-12 Revised:2017-01-12 Online:1985-08-31 Published:2017-01-12

摘要: 首次用肺吸虫成虫初步纯化抗原,进行PRAST测试肺吸虫病人的特异性IgG,阳性率为91.3%。健康人的假阳性率为3%。与ELISA比较(病人的阳性率为98.8%,正常人为0),PRAST的检出率略低。用PRAST检测肺吸虫病人特异IgG的灵敏度高于ELISA。

关键词: 肺吸虫病, 吸附试验, ELISA, 病人血清, 同位素标记物, 寄生虫病研究所, 抗原, 阳性率, 交叉反应, 肝吸虫病

Abstract: Paper radioantigensorbent test(PRAST) with preliminarily purified antigen of adult Paragonimus westermani was performed for the detection of specific antibodies in para gonimiasis and compared with ELISA.The positive rate of PRAST in 80 parasitologically confirmed paragoniraiasis cases was 91.3% with a false positive rate of 3% in 100 healthy individuals. By comparison, PRAST gave a slightly lower positive rate than ELISA for detecting antibodies in paragonimiasis, but exhibited higher titres in positive sera. No cross-reaction with sera from schistosomiasis was observed in both tests. Only very weak reaction occurred in PRAST with sera from clonorchiasis and fasciolopsiasis.