中国寄生虫学与寄生虫病杂志 ›› 1984, Vol. 2 ›› Issue (1): 43-45.

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外源凝集素对不同株利什曼原虫前鞭毛体凝集作用的初步观察

柴君杰,李宝山,王伟   

  1. 新疆维吾尔自治区卫生防疫站
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1984-02-28 发布日期:2017-01-12

AGGLUTINATION ACTIVITIES OF LECTINS ON VARIOUS STRAINS OF LEISHMANIA PROMASTIGOTES

  • Received:2017-01-12 Revised:2017-01-12 Online:1984-02-28 Published:2017-01-12

摘要: 初步观察了刀豆素A(Con A),植物血凝素(PHA),麦胚凝集素(WGA)和蓖麻凝集素(RCA)对5株杜氏利什曼原虫,1株墨西哥利什曼原虫亚马逊亚种,1株砂鼠利什曼原虫和1株蜥蜴利什曼原虫的凝集作用。Con A和RCA对8个虫株均有凝集作用,PHA和WGA均无作用,表明这8株利什曼原虫前鞭毛体细胞表面存在α-D甘露糖和/或α-D葡萄糖以及α-D半乳糖类成分。

关键词: 利什曼原虫, 凝集素, 前鞭毛体, 凝集作用, 初步观察, 细胞表面, D半乳糖, 酶处理, 感染能力, 杜氏利什曼

Abstract: The agglutination activities of concanavalin A, phytohemagglutinin, wheat germ agglutinin and Ricinus communis agglutiiiin on five strains of Leishmania donovani, one strain of L. mexicana amazonensis, one strain of L. gerbilae and one strain of L. sp. isolated from lizards were studied. Con A and RCA agglutinated specifically all the eight strains. Agglutination with PHA or WGA did not occur in any of the strains tested. These results showed the presence of terminal ligands as α-D mannose and/or a-D glucose and a-D galactose on the surface membrane of these strains of Leishmania.