广西地区艾滋病病毒感染/艾滋病患者蓝氏贾第鞭毛虫感染情况及基因型分析

doi:10.12140/j.issn.1000-7423.2019.03.014

中国寄生虫学与寄生虫病杂志 ›› 2019, Vol. 37 ›› Issue (3): 321-325.doi: 10.12140/j.issn.1000-7423.2019.03.014

广西地区艾滋病病毒感染/艾滋病患者蓝氏贾第鞭毛虫感染情况及基因型分析

刘华(), 徐宁, 沈玉娟^*(), 胡媛, 曹建平

中国疾病预防控制中心寄生虫病预防控制所,国家热带病研究中心,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海 200025

收稿日期:2019-04-01 出版日期:2019-06-30 发布日期:2019-07-10
通讯作者: 沈玉娟
作者简介:
作者简介：刘华（1986-）,女,博士研究生,从事肠道原虫检测及感染免疫研究。E-mail：liuhua2033@163.com
基金资助:
国家重点研发计划（No. 2017YFD0500400,No. 2016YFC1201900）,上海市卫生和计划生育委员会青年基金（No. 20164Y0225）,国家科技重大专项（No. 2018ZX10713001,No. 2018ZX10102001-002-004）,中国疾病预防控制中心青年基金（No. 2016A102）

Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi

Hua LIU(), Ning XU, Yu-juan SHEN^*(), Yuan HU, Jian-ping CAO

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention;Chinese Center for Tropical Diseases Research;WHO Collaborating Centre for Tropical Diseases;National Center for International Research on Tropical Diseases, Ministry of Science and Technology;Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China

Received:2019-04-01 Online:2019-06-30 Published:2019-07-10
Contact: Yu-juan SHEN
Supported by:
Supported by the National Key R&D Program of China (No. 2017YFD0500400, No. 2016YFC1201900）, Shanghai Municipal Commission of Health and Family Planning (No. 20164Y0225）, the National Science and Technology Major Program of China（No. 2018ZX10713001, No. 2018zx10102001-002-004）, the Project Sponsored by the Young Scholar Scientific Research Foundation of China CDC (No. 2016A102)

摘要/Abstract

摘要：

目的了解广西地区艾滋病病毒感染/艾滋病（HIV/AIDS）患者蓝氏贾第鞭毛虫（简称贾第虫）感染情况及基因型分析。方法收集广西地区HIV/AIDS患者和健康者粪样,镜检后提取粪样中贾第虫DNA,并采用巢式PCR方法扩增贾第虫磷酸丙糖异构酶基因（triosephosphate isomerase,TPI）,对阳性结果进行测序和BLAST比对分析,并构建进化树。结果共收集285例HIV/AIDS患者粪样,其中男性216例,女性69例,年龄21~85岁,其中130例曾接受抗逆转录病毒治疗。HIV/AIDS患者粪样镜检贾第虫阳性8份,阳性率为2.8%;健康者粪样303份,无贾第虫感染,两者差异有统计学意义（P < 0.01）。巢式PCR扩增阳性粪样,发现530 bp左右有一条特异性条带。经测序分析,有7份贾第虫样品为人兽共患集聚体B型,其中6份与已报到的人源分离株TPI基因序列一致性为100%。另外1份为集聚体C型,且该分离株与已报到的人源贾第虫集聚体C型基因序列一致性为100%。结论广西地区部分HIV/AIDS患者感染贾第虫,其基因型主要为集聚体B型。

关键词: 贾第虫, 集聚体, 巢式PCR, 艾滋病病毒感染/艾滋病

Abstract:

Objective To investigate the co-infection rate and the genotype of Giardia lamblia among HIV/AIDS patients in Guangxi Autonomous Region. Methods Fecal specimens were collected from 285 HIV/AIDS patients and 303 HIV-negative people from Guangxi Autonomous Region. DNA were extracted from the feces and triosephosphate isomerase (TPI) gene of G. lamblia was amplified by nested PCR to determine the infection of G. lamblia. The amplified TPI PCR products were sequenced and the obtained sequences were aligned and analyzed by BLAST search. Results The overall co-infection rate of G. lamblia with HIV/AIDS was 2.8% (8/285). There was no G. lamblia infection detected in people without HIV-AIDS (P < 0.01). However, no significant risk factors were found to be related to the infection of G. lamblia among HIV-AIDS patients such as age, gender, sanitation, antiretroviral treatment or clinical manifestations (diarrhea, CD4⁺T lymphocyte level). Seven samples were identified to be zoonotic assemblage B and 6 had 100% sequence homology with the reported human-derived isolate. The other isolate was assemblage C and sequence identical to the reported human-derived isolate in our previous study. Conclusion HIV-AIDS patients have higher co-infection rate with G. lamblia than people without HIV infection. The major genotype of infected G. lamblia was assemblage B.

Key words: Giardia lamblia, Assemblage, Nested PCR, HIV/AIDS

中图分类号:

R531.73

刘华, 徐宁, 沈玉娟, 胡媛, 曹建平. 广西地区艾滋病病毒感染/艾滋病患者蓝氏贾第鞭毛虫感染情况及基因型分析[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(3): 321-325.

Hua LIU, Ning XU, Yu-juan SHEN, Yuan HU, Jian-ping CAO. Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2019, 37(3): 321-325.

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参考文献 24

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风险因素 Risk factors	人数 No. of people	感染人数 G. lamblia infected	阳性率/% Positive rate/%	χ²值 χ² value	P值 P value
性别Gender
男Male	216	7	3.2	0.134	> 0.05
女Female	69	1	1.4
年龄组Age group
<40	93	1	1.1		> 0.05
40~60	113	4	3.5
>60	79	3	3.8
职业Occupation
农民 Farmer	217	7	3.2	0.118	> 0.05
其他 Others	68	1	1.5
CD4⁺ T细胞数
CD4⁺ lymphocyte count
CD4⁺ ≥ 200	49	1	2.0	0.052	> 0.05
CD4⁺ < 200	119	5	4.2
是否进行抗病毒治疗Antiretroviral treatment
是Yes	119	4	3.4	0	> 0.05
否No	131	4	3.1
感染途径Transmission route
性传播 Sexual	240	5	2.1	1.48	> 0.05
其他Others	45	3	6.7
饮用生水Drinking unboiled water
是Yes	19	0	0		> 0.05
否No	266	8	3.0
是否发生腹泻Diarrhea
是Yes	33	0	0		> 0.05
否No	252	8	3.2

广西地区艾滋病病毒感染/艾滋病患者蓝氏贾第鞭毛虫感染情况及基因型分析

Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi

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