Table of Content

31 May 1995, Volume 13 Issue 2

Previous Issue    Next Issue

A GREAT SUCCESS IN LYMPHATIC FILARIASIS CONTROL IN CHINA

1995, 13(2):  81-85. 

Asbtract ( )   PDF (153KB) ( )  

Related Articles | Metrics

Lymphatic filariasis control was first included in national program in 1956.Since then,a large scale anti filariasis campaign has been carried out extensively throughout the endemic areas.From 1956-1994,a cumulative total of 707 421 736 person time were comprised in nationwide blood examination,and a total of 260 041 645 person time DEC chemotherapy were conducted(including selective treatment,mass chemotherapy and DEC fortified salt).Up to 1994,all of the 864 endemic counties/cities in 15 provinces/autonomous regions/municipalities had achieved effective control of filariasis successively.Parasitological,entomological and serological surveillance during the past few years demonstrated that the transmission of both bancroftian and malayan filariasis have been virtually interrupted in most of the areas reaching the criterion for effective control of filariasis.

EXPRESSION OF CHEMICALLY SYNTHESIZED HYBRID GENES OF PLASMODIUM FALCIPARUM AND PRELIMINARY CHARACTERIZATION OF EXPRESSED PRODUCTS

1995, 13(2):  94-98. 

Asbtract ( )   PDF (183KB) ( )  

Related Articles | Metrics

The chemically synthesized hybrid genes HGFC and HGFCAC were respectively recombinated with expression vector pWR450 1 and transferred into Escherichia coli .After adding IPTG to the cultural media,the bacteria harboring recombinant plasmid expressed the fusion proteins of exogenous gene products and partial amino acid of β galactosidase.The molecular weights of the two fusion proteins were 65 kDa and 77 kDa,respectively.The expressed fusion proteins could be recognized by antibodies of rabbit anti RESA peptide EENVEHDA by Western blotting,indicating that the fusion proteins harbored the antigenic epitopes of Plasmodium falciparum .

NATIONWIDE SURVEY OF THE DISTRIBUTION OF PARASITES IN CHINA THE CHARACTERISTICS AND RULES OF GEO GRAPHIC DISTRIBUTION OF HUMAN HELMINTH INFECTION

1995, 13(2):  99-103. 

Asbtract ( )   PDF (185KB) ( )  

Related Articles | Metrics

There are obvious characteristics and rules of the geographic distribution of human helminth infection in China,such as the distribution of trematode infection is accompanied with the river system valley,the distribution of cestode infection differs in the terrain with three classes of stairs,and the distribution of soil helminth infection accords with the tempe rature zone and areas with different humidity.

OBSERVATION ON THE DEVELOPMENT OF COELOMOMYCES INDICA IN CULEX TRITAENIORHYNCHUS LARVAE 

1995, 13(2):  104-106. 

Asbtract ( )   PDF (161KB) ( )  

Related Articles | Metrics

The present paper reports the development of Coelomomyces indica in Culex tritaeniorhynchus larvae.Experimental observations indicated that the first instar of the mosquito larvae can be infected with fungus.The hyphal bodies developed and produced sporangia in the haemocoele of the mosquito larvae.The sporangia were released in water from the dead mosquito larvae.The development of mature sporangia with zoospores was in water at 34℃ within 8 12 hours.The zoospores released from the mature sporangia within 1/2 1 hour under optimal conditions were very active in water and their activities decreased after 1 hour.The zoospores act as the infective stage for the Cyclops (host).

EFFECTS OF MEBENDAZOLE, ALBENDAZOLE AND PRAZIQUANTEL ON ALANINE AMINOTRANSFERASE AND ASPARTATE AMINOTRANSFERASE OF ECHINOCOCCUS GRANULOSUS CYST WALL HARBORED IN MICE 

1995, 13(2):  107-109. 

Asbtract ( )   PDF (132KB) ( )  

Related Articles | Metrics

Alanine aminotransferase(ALT) and aspartate aminotransferase (AST) of Echinococcus granulosus cyst wall harbored in mice for 10-12 months was measured.The activities of the 2 enzymes were 3 430±1 370-4 160±1 790 and 1 560±840-2 890±1 470,respectively.When infected mice were treated ig with mebendazole(Meb)25 mg·kg -1 ·d -1 for 7-14 d,or 50 mg·kg -1 ·d -1 for 7 d,the ALT activities of cyst walls of both collapsed and full cysts were inhibited significantly with average inhibition rates of about 50%。Another benzimidazole derivative,albendazole(Alb),also inhibited ALT activity of the cyst wall with an inhibition rate of 40.7%,but this was not the case in the treatment with praziquantel 500 mg·kg -1 ·d -1 of the infected mice for 14 d.When the infected mice were treated ig with the above mentioned drugs at the same regimens,no apparent effect on AST activities of the cyst wall was seen except for the group treated ig with Meb at a higher daily dose,the inhibition rates being 26%(collapsed cyst)and 43.9%(full cyst).The significance of the results has been discussed briefly.

MOLLUSCICIDAL ACTIVITY OF SEED OF CAMELLIA SINENSIS

1995, 13(2):  114-116. 

Asbtract ( )   PDF (109KB) ( )  

Related Articles | Metrics

This paper is a preliminary report on the molluscicidal activity of seed of Camellia sinensis (L) O. Kuntze (SCS).Its LD 50 and LD 90 were 10 and 16 μg/ml,respectively.We found also SCS inhibited oxygen consumption 65% and glycogen content of Oncomelania snail by 80%.The activity of hatching eggs to juvenile snails was inhibited by this seed at 8-16 μg/ml.Juvenile snails may also be killed by this molluscicide.

A PRELIMINARY STUDY ON THE INHIBITORY ACTION OF TARGET PRIMAQUINE ON THE EXOERYTHROCYTIC FORMS OF PLASMODIUM YOELII YOELII

1995, 13(2):  117-119. 

Asbtract ( )   PDF (165KB) ( )  

Related Articles | Metrics

Target primaquine(TPQ) the primaquine entrapped by galactosylneoglycoalbumin(G NGA),was given intravenously(iv)to Wistar rats 2 h after sporozoite inoculation.The sporozoite viability(SPV) of TPQ 20 mg/kg group was revealed to be 1.2% 42 h after inoculation,which showed a decline of about 6% as compared to the control;besides,degeneration or retardation of development was present in 90% of EEF.In TPQ 10 mg/kg and PQ 20 mg/kg groups,the SPV were 2.8% and 5.0%, respectively,showing drops by 15% and 26% as compared with that of the control.Degeneration or retardation in development was also visible in about half of the EEF.In mice which were treated iv with TPQ 10 mg/kg or PQ 20 mg/kg 2 h after sporozoite inoculation,the prepatent periods were delayed markedly as compared with that of the control.Parasitemia did not appear in 2 mice in 10 mg/kg TPQ group further indicated the effectiveness of the drug.The results suggest that the anti EEF effect of TPQ is apparently more promising than that of PQ.

APPLICATION OF PLASMODIUM FALCIPARUM DNA EXTRACT FROM DRIED BLOOD SPOT OF FALCIPARUM MALARIA PATIENT IN POLYMERASE CHAIN REACTION

1995, 13(2):  120-126. 

Asbtract ( )   PDF (113KB) ( )  

Related Articles | Metrics

Polymerase chain reaction (PCR) primered with primers 3,4 and 5,6 was performed using DNA extracted from dried blood spot of a falciparum malaria patient from Mengpeng Township,Yunnan using four different methods,including STE proteinase K SDS,methanol proteinase K SDS,Chelex 100 and Chelex 100 proteinase K to amplify DNA of apical membrane antigen 1(AMA 1).A 900 bp DNA band could be seen on the ethidium bromide stained agarose gel electrophoresis of the PCR products when DNA was extracted using all the above mentioned methods except STE proteinase K SDS method. DNA extracted with Chelax 100 was recommended.

EFFECT OF CLADONIA ALPESTRIS ON TRICHOMONAS VAGINALIS IN VITRO

1995, 13(2):  130-133. 

Asbtract ( )   PDF (134KB) ( )  

Related Articles | Metrics

In this paper, an experimental research is reported on the effect of water extract of Cladonia alpestris and S-(-)usnic acid on Trichomonas vaginalis in vitro. The results showed that both the water extract of Cladonia alpestris and S-(-)usnic acid exhibited a strong effect against Trichomonas vaginalis in vitro .As the time of action of the agents was prolonged , the mortality of Trichomonas vaginalis increased.For S-(-)usnic acid, 0.4 mg/ml was the lowest effective concentration against Trichomonas vaginalis in vitro .No remarkable differences were found in the effect of the S-(-)usnic acid and metronidazole at concentrations of 0.4 mg/ml and 0.6 mg/ml.

EXPERIMENTAL AND CLINICAL STUDY ON PNEUMOCYSTOSIS Ⅳ USE OF A 4D7 MONOCLONAL ANTIBODY TO DETECT PNEUMOCYSTIS CARINII BY IMMUNOPEROXIDASE STAINING

1995, 13(2):  134-137. 

Asbtract ( )   PDF (185KB) ( )  

Related Articles | Metrics

This paper reports on the diagnostic utility of the 4D7 monoclonal antibody (McAb) and immunohistochemical (IHC) staining to detect P.carinii. Specimens of 30 pathological sections of lung tissues and 1 smear of aspirated bronchial material from confirmed cases,and 12 sputum smears from high risk patients with pneumonia were examined by immunohistochemical method using the 4D7 McAb.The results showed that both the sensitivity and specificity of the 4D7 McAb IHC staining were 100% (31/31).A comparison between immunohistochemical staining with GMS and TBO staining was made,no difference in sensitivity was found.However,the IHC method gave a clear back ground that is useful for definite diagnosis.

RELATIONSHIP BETWEEN TUMOUR NECROSIS FACTOR AND ANEMIA OF MALARIA

1995, 13(2):  138-141. 

Asbtract ( )   PDF (133KB) ( )  

Related Articles | Metrics

In this paper,the relation between the level of the reactive oxygen species(ROS) and relevant free radicals in the blood plasma of the BALB/c mice infected with Plasmodium berghei ANKA strain and their erythrocytic deformability(ED),and the relation between the ED and the Hb indices of these mice were studied by chemiluminescence(CL),induced CL(ICL) and laser diffraction method.The results indicated that the ED decreased with the increase of the level of ROS and free radicals in blood plasma when the anemia is developing.The use of antioxidants may restrain the course and raise the Hb index.The authors deem that the excess TNF produced in the later stage of malaria may induce the release of excess ROS and free radicals from phagocytes,which may cause damage on the deformability of erythrocytes and a drop in ED,resulting in the anemia of malaria.

EXPLORATION ON EXPERIMENTAL CONDITIONS OF DOT ELISA FOR TRICHOMONAS VAGINALIS USING INTACT PARASITE ANTIGEN MONOCLONAL ANTIBODY DETECTING SYSTEM

1995, 13(2):  142-145. 

Asbtract ( )   PDF (163KB) ( )  

Related Articles | Metrics

This paper deals with the effect of different experimental conditions including the number of parasite, blocking fluid, incubation time and different types of antigens on dot ELISA for Trichomonas vaginalis. Five microlitres of freshly washed intact worms at a concentration of 7.7×10 5-3×10 8 cells/ml were added onto nitrocellulose membrane blocked with 1% BSA, 10% bovine serum or milk power (0.1%,0.25%,0.5%,5%). Dot ELISA using nitrocellulose membrane containing whole cell antigen 2.5×10 7 cells/ml, blocked by 0.5% defatted milk power at 37℃ for one hour before the incubation in McAb, incubated at 37℃ for one hour in McAb and SPA respectively was found to give more promising results. The whole cell antigen could be preserved for four months at 4℃ and -20℃ .