中国寄生虫学与寄生虫病杂志

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皖浙两省部分地区宠物犬肠道中犬新孢子虫的感染情况

李文超,刘德义,周力,曹佳彤,梅楠,张衡,陈程,陈涛,韩敏敏,张威,凡自来,顾有方*   

  1. 安徽科技学院动物科学学院,凤阳 233100
  • 出版日期:2015-08-30 发布日期:2015-09-10

Prevalence of Neospora caninum Infection in the Intestine of Pet Dogs in Some Areas of Anhui and Zhejiang

LI Wen-chao, LIU De-yi, ZHOU Li, CAO Jia-tong, MEI Nan, ZHANG Heng, CHEN Cheng, CHEN Tao, HAN Min-min, ZHANG Wei, FAN Zi-lai, GU You-fang*   

  1. College of Animal Science,Anhui Science and Technology University, Fengyang 233100, China
  • Online:2015-08-30 Published:2015-09-10

摘要:

目的  了解安徽省和浙江省宠物犬肠道中犬新孢子虫的感染情况。  方法  2013年4~12月从安徽省合肥市包河区、宣城市宣州区、滁州市凤阳县和明光市、蚌埠市龙子湖区和宿州市泗县,以及浙江省杭州市余杭区的宠物门诊采集315份新鲜宠物犬粪样。所有样品处理后先采用基于犬新孢子虫特异基因NCLI-004830的巢式PCR方法进行检测,随后对阳性样品进行PCR扩增和分析其内转录间隔区1(ITS1)序列以进一步验证。  结果  315份粪样中NCLI-004830基因巢式PCR共扩增出5份犬新孢子虫DNA阳性样品,产物大小为420 bp,阳性率为1.6%,滁州和蚌埠宠物犬的阳性率分别为3.4%(3/89)和6.5%(2/31),其余调查点未发现犬感染犬新孢子虫。5份NCLI-004830基因阳性样品的ITS1序列经PCR扩增后,均扩增出137 bp的条带,序列鉴定正确。成年犬和幼龄犬之间,雄性犬和雌性犬之间差异均无统计学意义(P>0.05)。  结论  安徽省滁州和蚌埠的宠物犬有犬新孢子虫感染。

关键词: 犬新孢子虫, 巢式PCR, 宠物犬, 内转录间隔区1, 安徽, 浙江

Abstract:

Objective  To investigate the prevalence of Neospora caninum infection in the intestine of pet dogs in areas of Anhui and Zhejiang.  Methods  A total of 315 fecal samples from pet dogs were collected in pet clinics from April to December 2013 in Baohe District in Hefei city, Xuanzhou District in Xuancheng city, Fengyang County in Chuzhou city, Longzihu District in Bengbu city, and Si County in Suzhou City in Anhui Province, as well as in Yuhang District in Hangzhou city of Zhejiang province. All samples underwent nested-PCR targeting Neospora-specific gene NCLI-004830. The results were further confirmed by PCR amplification of N. caninum ITS1 followed by sequence analysis.  Results  The rate of N. caninum infection in the 315 samples was 1.59%(5/315). The infection rate in Chuzhou and Bengbu was 3.37% and 6.45%, respectively, and no N. caninum infection was found in the remaining areas. There was no association between the infection rate and the sex or age of the dogs.  Conclusion  N. caninum infection is prevalent in pet dogs in Chuzhou and Bengbu of Anhui.

Key words: Neospora caninum, Nested-PCR, Pet dog, ITS1, Anhui, Zhejiang