棘球蚴感染绵羊并诱发休克期间特异性IgG、IgE抗体对特异性抗原的识别

中国寄生虫学与寄生虫病杂志 ›› 2002, Vol. 20 ›› Issue (6): 12-360.

棘球蚴感染绵羊并诱发休克期间特异性IgG、IgE抗体对特异性抗原的识别

郑宏,徐志新,杨戈雄,温浩

新疆医科大学第一附属医院麻醉科;新疆医科大学第一附属医院麻醉科;武警新疆总队医院麻醉科;新疆医科大学第一附属医院普外科乌鲁木齐 830054;乌鲁木齐 830054;乌鲁木齐 830091;乌鲁木齐 830054

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-12-30 发布日期:2002-12-30

Recognition of Specific Antigens by Specific IgG and IgE During Anaphylactic Shock Induced by Echinococcus granulosus in Sheep

ZHENG Hong;XU Zhi-xin;YANG Ge-xiong;WEN Hao

1 Department of Anesthesiology;First Affiliated Hospital of Xinjiang Medical University;Urumqi 830054;2 Department of Anesthesiology;Xinjiang Corps Hospital of Chinese People's Armed Police Forces;Urumqi 830054;3 Department of General Surgery;First Affiliated Hospital of Xinjiang Medical University;Urumqi 830054

Received:1900-01-01 Revised:1900-01-01 Online:2002-12-30 Published:2002-12-30

摘要/Abstract

摘要： 　　目的探讨细粒棘球蚴(E.g.)囊液粗制抗原和B抗原(EgB)识别绵羊感染E.g.后及诱发过敏性休克期间特异性IgG和IgE抗体的特异性反应,并对抗原特性及分子量进行描述。方法制备E.g.囊液粗制抗原及EgB,应用免疫印迹技术检测经剖杀证实感染E.g.并诱发过敏性休克的20只绵羊血清特异性IgG和IgE抗体对两种抗原的抗体反应性,并对抗原特性和分子量进行描述。结果特异性IgE抗体与耐热、低分子量的EgB(8、12和16 kDa)抗原未见明显的反应条带,而与E.g.囊液粗制抗原在43 kDa处可见明显的反应条带。IgG抗体与E.g.囊液粗制抗原未见明显的反应条带,但与EgB抗原反应后在31、43和66.2 kDa处可见较为明显的反应条带。结论 EgB可能不是特异性IgE抗体的特异性抗原,而是IgG抗体的特异性抗原。E.g.粗制囊液抗原中含有特异性IgE抗体的特异性抗原组分,其分子量大于43 kDa,可能是导致棘球蚴病患者过敏性休克的主要致敏原。

关键词: 细粒棘球蚴囊液粗制抗原, EgB抗原, 免疫印迹技术

Abstract: 　Objective To determine the specific recognition of Echinococcus granulosus (E.g.) cyst fluid crude antigen (EgCF) and antigen B (EgB) by serum specific IgG and IgE using Western blotting during anaphylactic shock induced by E. g. in sheep, and to investigate the importance of defined characteristics and molecular weight of the specific antigens. Methods EgCF was obtained through local slaughterhouses in Urumqi from the cysts of infected sheep liver. Western blotting was used to analyze total specific IgG and IgE antibodies in serum from sheep infected with E.g. using either crude antigen of E. g. and EgB, and to understand specific recognition of hydatid cyst antigens by serum total IgG and specific IgE. Results SDS-PAGE and Western blotting showed that there were differences between EgB and EgCF in electrophoresis pattern. EgB was recognized by IgG from sera of infected sheep in a series of bands with molecular weight ranging from 31, 43 to 66.2 kDa. No binding of IgG against EgCF was observed in any serum from the infected sheep during anaphylactic shock. In contrast, specific IgE antibodies in E. g. infected sheep obviously recognized the single 43 kDa subunit of EgCF, but no binding of specific IgE against EgB was observed in sera of the infected sheep. Conclusion EgCF is consisted of antigenic components in which there is a specific antigen against IgE with a molecular weight of over 43 kDa. This component may lead to an anaphylactic shock induced by E. g. . EgB is a specific antigen against the total IgG but not to the specific IgE.

Key words: Echinococcus granulosus cyst fluid crude antigen, E. g antigen B, Western blotting

郑宏;徐志新;杨戈雄;温浩. 棘球蚴感染绵羊并诱发休克期间特异性IgG、IgE抗体对特异性抗原的识别[J]. 中国寄生虫学与寄生虫病杂志, 2002, 20(6): 12-360.

ZHENGHong;XUZhi-xin;YANGGe-xiong;WENHao. Recognition of Specific Antigens by Specific IgG and IgE During Anaphylactic Shock Induced by Echinococcus granulosus in Sheep[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2002, 20(6): 12-360.

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