新疆察布查尔县小型啮齿类动物多房棘球蚴感染情况调查

doi:10.12140/j.issn.1000-7423.2021.03.006

中国寄生虫学与寄生虫病杂志 ›› 2021, Vol. 39 ›› Issue (3): 327-332.doi: 10.12140/j.issn.1000-7423.2021.03.006

新疆察布查尔县小型啮齿类动物多房棘球蚴感染情况调查

郭宝平¹(), 郭刚¹, 张莉², 向晶晶³, 王小平⁴, 任远⁵, 齐文静⁵, 张慧⁶, 李军¹, 张文宝¹, 汪海燕²^,^*()

1 新疆医科大学第一附属医院临床医学研究院,乌鲁木齐 830011
2 新疆察布查尔县人民医院,察布查尔县 835300
3 新疆察布查尔县疾病预防控制中心,察布查尔县 835300
4 新疆伊犁州疾病预防控制中心,伊宁 835000
5 新疆医科大学省部共建中亚高发病成因与防治国家重点实验室,乌鲁木齐 830011
6 新疆医科大学基础医学院,乌鲁木齐 830011

收稿日期:2020-12-22 修回日期:2021-01-21 出版日期:2021-06-30 发布日期:2021-07-05
通讯作者: 汪海燕
作者简介:郭宝平（1979-）,男,博士,助理研究员,主要从事寄生虫免疫学及遗传学研究。E-mail： 1239061976@qq.com
基金资助:
国家自然科学基金(81830066);国家自然科学基金(U1803282);国家自然科学基金(31860703)

Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang

GUO Bao-ping¹(), GUO Gang¹, ZHANG Li², XIANG Jing-jing³, WANG Xiao-ping⁴, REN Yuan⁵, QI Wen-jing⁵, ZHANG Hui⁶, LI Jun¹, ZHANG Wen-bao¹, WANG Hai-yan²^,^*()

1 The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
2 Chabuchaer County People’s Hospital, Chabuchaer 835300, China
3 Chabuchaer Center for Disease Control and Prevention, Chabuchaer 835300, China
4 Yili Prefecture Center for Disease Control and Prevention, Yining 835000, China
5 State Key Laboratory of Pathogenesis, Prevention and Treatment of Central Asian High Incidence Diseases, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
6 Basic Medical College of Xinjiang Medical University, Urumqi 830011, China

Received:2020-12-22 Revised:2021-01-21 Online:2021-06-30 Published:2021-07-05
Contact: WANG Hai-yan
Supported by:
National Natural Sciecne Foundation of China(81830066);National Natural Sciecne Foundation of China(U1803282);National Natural Sciecne Foundation of China(31860703)

摘要/Abstract

摘要：

目的了解新疆察布查尔县多房棘球绦虫流行区中间宿主——小型啮齿类动物的感染和分布情况,确定多房棘球绦虫的中间宿主种类,为当地制定防控措施提供科学依据。方法 2019年选择加尕斯台乡、琼博乐乡、四师和都拉塔口岸等4个调查点,使用板夹、弓形夹捕捉小型啮齿类动物。根据外形特点并结合头骨形态鉴定小型啮齿类动物的种类。取捕获的小型啮齿类动物疑似感染的肝脏分别进行HE染色及PCR方法扩增并测序确定其感染情况。不同地区和不同种群小型啮齿类动物感染率的比较采用χ²检验。结果本调查共捕获小型啮齿类动物595只,经形态学鉴定分属7个种,其中普通田鼠464只、小林姬鼠84只、柽柳沙鼠14只、大沙鼠14只、坦氏鼹形田鼠8只、灰仓鼠6只和红尾沙鼠5只。67份疑似感染的肝脏样品可见多房棘球蚴病灶,HE染色结果显示,病灶组织可见多房棘球蚴原头节;PCR扩增结果和序列比对表明,该67只小型啮齿类动物均为多房棘球蚴感染,总感染率为11.3%。各种的感染率分别为普通田鼠12.8%（57/464）、小林姬鼠3.6%（3/84）、柽柳沙鼠1/14、大沙鼠2/14、坦氏鼹形田鼠2/8、红尾沙鼠1/5和灰仓鼠1/6。普通田鼠的感染率与小林姬鼠比较差异有统计学意义（P < 0.05）;普通田鼠与其他5种小型啮齿类动物之间感染率差异无统计学意义（ P > 0.05）。4个调查点中,以琼博乐乡捕获的小型啮齿类动物的感染率最高,为12.3%（49/398）。结论新疆察布查尔县所捕获的小型啮齿类动物主要为普通田鼠和小林姬鼠,前者多房棘球蚴感染率最高。

关键词: 多房棘球蚴, 多房棘球绦虫, 小型啮齿类动物, 普通田鼠, 新疆察布查尔县

Abstract:

Objective To investigate the infection and distribution of Echinococcus multilocularis in small rodents, the intermediate hosts, in Chabuchaer County, Xinjiang, and to identify the intermediate host species of the parasite, providing scientific basis for formulating local control measures. Methods Small rodents were captured with plate clamps and bow clips in four selected investigation sites, including Jiagasitai town, Qiongbole town, Sishi and Dulata Port. Suspected infection in liver tissues was confirmed by HE staining and PCR amplification. The PCR products were sequenced. The species of small rodents were identified according to their physical appearance and skull morphology. Theχ² test was used to compare the infection rate in different regions and in different rodent populations. Results A total of 595 small rodents were captured, which belonged to 7 species, including 464 Microtus spp., 84 Apodemus sylvaticus, 14 Meriones tamariscinus Pallas, 14 Rhombomys sopimus Pallas, 8 Ellobius trancrei, 6 Cricetulus migratorius Pallas and 5 Meriones erythrourus Pallas. In 67 liver samples of suspected infection, lesions of E. multilocularis metacestodes were observed. HE staining showed that the protoscoleces of E. multilocularis metacestodes were visible in the lesion tissues. PCR amplification and sequence alignment showed that all the 67 small rodents were infected with metacestodes of E. multilocularis, with a total infection rate of 11.3%. All the seven species of rodents were infected, with infection rates of 12.8% (57/464), 3.6% (3/84), 1/14, 2/14, 2/8, 1/5 and 1/6 for Microtus spp, A. sylvaticus, M. tamariscinus Pallas, R. sopimus Pallas, E. trancrei, M. serythrourus Pallas and C. migratorius Pallas, respectively. Chi-square analysis showed that there was a significant difference in the infection rate between Microtus spp. and A. sylvaticus (P < 0.05). The infection rate of Microtus spp. did not significantly differ from that of the other species (P > 0.05). Of the 4 investigation sites, the Qiongbole town showed the highest infection rate (12.3%, 49/398). Conclusion The small rodents captured in Chabuchaer County were mainly Microtus spp. and A. sylvaticus, with the former species showing the highest infection rate of metacestodes.

Key words: Metacestodes of Echinococcus multilocularis, Echinococcus multilocularis, Small rodent, Microtus spp., Chabuchaer County, Xinjiang

中图分类号:

R532.32

郭宝平, 郭刚, 张莉, 向晶晶, 王小平, 任远, 齐文静, 张慧, 李军, 张文宝, 汪海燕. 新疆察布查尔县小型啮齿类动物多房棘球蚴感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(3): 327-332.

GUO Bao-ping, GUO Gang, ZHANG Li, XIANG Jing-jing, WANG Xiao-ping, REN Yuan, QI Wen-jing, ZHANG Hui, LI Jun, ZHANG Wen-bao, WANG Hai-yan. Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2021, 39(3): 327-332.

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种属Species	加尕斯台乡 Jiagasitai Town		琼博乐乡 Qiongbole Town		四师Sishi		都拉塔口岸Dulata port		合计Total
种属Species	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)	捕获只数 No. of captures	感染只数（感染率/%）No. positive(Infection rate/%)
普通田鼠 Microtus spp.	71	8（11.3）	366	46（13.9）	19	2（2/19）	8	1（1/8）	464	57（12.8）
坦氏鼹形田鼠 E. trancrei	0	0	8	2（2/8）	0	0	0	0	8	2（2/8）
小林姬鼠 A. sylvaticus	23	1（4.4）	24	1（4.2）	17	1（1/17）	20	0	84	3（3.6）
柽柳沙鼠M. tamariscinus Pallas	0	/	0	/	7	0	7	1（1/7）	14	1（1/14）
大沙鼠 R. sopimus Pallas	0	/	0	/	7	1（1/7）	7	1（1/7）	14	2（2/14）
红尾沙鼠 M. erythrourus Palla	0	/	0	/	0	/	5	1（1/5）	5	1（1/5）
灰仓鼠 C. migratorius Pallas	0	/	0	/	0	/	6	1（1/6）	6	1（1/6）
合计Total	94	9（9.6）	398	49（12.3）	50	4（8.0）	53	5（9.4）	595	67（11.3）

新疆察布查尔县小型啮齿类动物多房棘球蚴感染情况调查

Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang

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