关键词: 日本血吸虫, 童虫, 体外培养方法, 腹吸盘, 抗血吸虫药物, 生长发育特点, 兔红细胞, 生长发育速度, 发育情况, 口吸盘

Abstract: This paper deals with some features of growth and development of three-day-old schistosomula of Schistosoma japonicum recovered from lungs of experimentally infected mice in both medium A and medium B. In young schistosomula, pigment appeared on the third day and cecal conjunction began on day 12. The degree of their development reached stage 3 (caeca united) by day 15. However, it occurred somewhat later as compared with development in vivo. About 50% of the schistosomula cultured in medium B can survive for at least 30 days and their size is five times as big as that at the beginning and also the optimal development reached stage 4 (organogeny).The in vitro procedures presented here for cultivating schistosomula might be used for the screening of antischistosomal drugs as well as for immunological, physiological and biochemical studies of Schistosoma japonicum.Finally, criteria are proposed to evaluate the effect of the in vitro cultivation of lung-phase schistosomula.