中国寄生虫学与寄生虫病杂志 ›› 1985, Vol. 3 ›› Issue (2): 122-124.

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贾第虫滋养体的扫描电镜观察

杜之鸣,李向印,张玉英,王丽   

  1. 河北医学院寄生虫学教研组; 河北医学院电镜室; 河北医学院电镜室; 河北医学院电镜室
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1985-05-31 发布日期:2017-01-12

SCANNING ELECTRON MICROSCOPY ON THE TROPHOZOITE OF GIARDIA LAMBLIA

  • Received:2017-01-12 Revised:2017-01-12 Online:1985-05-31 Published:2017-01-12

摘要: 分离、浓缩贾第虫包囊,人工感染家兔获得滋养体。扫描电镜观察滋养体呈倒置梨形,12~16.5×6.5~7.7μm。前端钝圆,后端尾部变细。背面隆起,表面呈桔皮样。前端腹面有一个不对称的螺旋形腹吸盘。虫体周缘具有突出的伪足样周翼,其背腹面均光滑,该翼与腹吸盘侧嵴和虫体背面之间分别形成边缘沟。滋养体有4对鞭毛,均由背侧边缘沟伸出体外。

关键词: 滋养体, 扫描电镜观察, 贾第虫, 腹吸盘, 小肠微绒毛, 中心区, 边缘沟, 小肠粘膜, 小肠绒毛, 前鞭毛

Abstract: The trophozoites of Giardia lamblia in mucosal scrapings and intestinal mucus obtained from the small intestine of experimentally infected rabbit were isolated, further purged from contaminating intestinal debris by alternating temperature cycles, and examined with scanning electron microscope.Trophozoites were inverted pear-shaped, approximately 12-16.5μm long and 6.5-7.7μm wide, with a tangerine skin-like dorsal surface. The ventral sucker was an asymmetric spiral structure on the ventral surface of the anterior end of the trophozoite, or rather, it appeared to be a clockwise spiraling structure starting from its central circular region. The central region of the ventral disc was as uneven as the dorsal surface but the other surfaces of the ventral disc were smooth. A pseudopod-like peripheral wing projecting from the peripheral edge of cell body with a smooth surface was observed forming ventral and dorsal marginal grooves respectively with the lateral crest, and the dorsal surface of cell body. The trophozoite has four pairs of flagella emerging from the dorsal marginal groove.