关键词: 日本血吸虫, SDS-聚丙烯酰胺凝胶电泳, 品系

Abstract: The purpose of the present study was to investigate the SDS-PAGE separation of proteins of the 5 different isolates of Schistosoma japonicum from the mainland of China to determine their similarities and/or differences and to gain additional data which could give an added insight into the degree of relationship.Soluble proteins of freshly sonicated adult worm extract of the 5 different isolates (i.e..Anhui,Hubei,Guangxi,Sichuan and Yunnan) were compared by SDS-polyacrylamide gel electrophoresis.The results indicated that no marked difference could be observed among the isolates after gels were stained by Coomassie blue while both male and female worms of Guangxi isolate showed some definite differences in their protein profile,i.e.,Jack of 5075 kDa band in male worms and a marked reduction in the quantity of 110 and 30 kDa bands in female worms as shown by silver stain.