关键词: —kDa抗原, 重组Sj抗原, 日本血吸虫

Abstract: This paper reports on the comparison of the recombinant Sj26 (rSj26) antigen originated from the Philippine strain and 24-26 kDa antigen isolated and purified from Chinese mainland strain of S.japonicum for their antigenicity and immunogenicity.The results showed that there were obvious cross reactions between rSj26 and 24-26 kDa anitigen when rSj26 antigen was tested against specific antibodies in sera from mice infected with the mainland strain of S.japonicum or 24-26 kDa antigen was tested against specific anti-rSj26 antibodies by ELISA,IFA and Western blotting etc.Both of 24-26 kDa and rSj26 antigen had weak cross reaction With SEA antigen.The worm reduction rate after challenging with mainland strain cercariae in mice immunized with rSj26 was 26~32%,similar to that in mice immunized with 24-26 kDa antigen.It is suggested that rSj26 antigen can induce certain level of specific protective immunity to protect the host against infection by Chinese strain of S.japonicum cercaciae.