中国寄生虫学与寄生虫病杂志 ›› 1993, Vol. 11 ›› Issue (3): 201-203.

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我国4省、自治区细粒棘球绦虫DNA限制性片段长度多态性分析

薛海筹,陈伟,裘丽姝,诸陈文   

  1. 中国预防医学科学院寄生虫病研究所
  • 出版日期:1993-08-31 发布日期:2017-01-06
  • 基金资助:
    国家自然科学基金

RFLP ANALYSIS OF DNA FROM ECHINOCOCCUS GRANULOSUS COLLECTED FROM FOUR PROVINCES/AUTONOMOUS REGION IN CHINA

  • Online:1993-08-31 Published:2017-01-06

摘要: 运用 DNA探针pHD5、pEG18、pSM889 结合 Southern blot 杂交技术对采自我国新疆、青海、甘肃和宁夏等地的羊源细粒棘球蚴与采自青海、甘肃的牦牛源细粒棘球蚴进行了限制性片段长度多态性(RFLP)分析。结果表明我国这些省、自治区采集的羊源细粒棘球蚴彼此之间未见有明显而稳定的杂交条带差异,属于同一虫株。青海和甘肃两省牦牛源细粒棘球蚴彼此之间也未见明显而稳定杂交条带差异,据杂交图谱判断似与羊源细粒棘球蚴属同一虫株。

关键词: 包虫病, 细粒棘球绦虫, DNA探针, 限制性片段长度多态性分析, 虫株

Abstract: A fragment of the ribosomal RNA gene of Schistosoma mansoni pSM889 and two DNA fragments specific to Echinococcus granulosus pHD5 and pEGlS have been used as DNA probes to assess the extent of genetic variability within E. granulosus. The DNA analysis,including restriction endonuclease digestion and Southern blot hybridization with the probes , did not demonstrate any genetic variation among E. granulosus collected from sheep in Xinjiang, Qinghai,Gansu and Ninxia. Similarly,there was no genetic variation among E. granulosus isolates collected from yak in Qinghai and Gansu provinces. The authors deemed that the yak isolates and the sheep isolates of E. granulosus appear to belong to a same strain.