关键词: 日本血吸虫, 虫卵, 6三硝基苯磺酸, 小鼠, 结肠炎

Abstract: Objective To study the suppression of Schistosoma japonicum eggs against the trinitrobenzene sulfo-nic acid （TNBS）-induced colitis in mice. Methods 50 female BALB/c mice （6-8 week-old） were randomly divided into normal control group， ethanol control group， schistosome egg immunized control group， TNBS-induced colitis group and TNBS-induced colitis with egg immunization group. In TNBS-induced colitis with egg immunization group， mice were immunized 4 times with 10 000 schistosome eggs by intraperitoneal injection with one-week interval. On day 6 after the last immunization the mice were induced by TNBS and the body weight， histological change on colon and level of cytokines of mice were observed in egg-immunized and -unimmunized colitis mice. Results The unimmunized mice developed significant inflammation in colon with bloody mucus feces and decreased body weight after TNBS induction. Distinct hyperemia， edema and transmural inflammatory infiltration accompanied with ulceration were shown in colon. The level of IFN-γ was （3.47±0.87） ng/ml and IL-4 was （146.06±45.76） pg/ml. However， in egg-immunized mice， the inflammation was suppressed greatly and the body weight recovered soon after TNBS induction. The production of IL-4 was enhanced to （598.50±135.90） pg/ml， and IFN-γ was significantly diminished to （1.53±0.51） ng/ml. Conclusion S. japonicum eggs protect mice from colitis induced by TNBS.

Key words: Schistosoma japonicum, Egg, Trinitrobenzene sulfonic acid （TNBS）-Mice, Colitis