疟原虫全基因组测序研究进展及其应用

doi:10.12140/j.issn.1000-7423.2021.02.021

中国寄生虫学与寄生虫病杂志 ›› 2021, Vol. 39 ›› Issue (2): 265-270.doi: 10.12140/j.issn.1000-7423.2021.02.021

疟原虫全基因组测序研究进展及其应用

刘宏¹^,²(), 刘耀宝², 曹俊¹^,²^,^*()

1 南京医科大学公共卫生学院全球健康中心,南京 211166
2 江苏省血吸虫病防治研究所,世界卫生组织消除疟疾研究与培训合作中心,国家卫生健康委员会寄生虫病预防与控制技术重点实验室,江苏省寄生虫与媒介控制技术重点实验室,无锡 214064

收稿日期:2020-08-18 修回日期:2021-01-15 出版日期:2021-04-30 发布日期:2021-04-30
通讯作者: 曹俊
作者简介:刘宏（1995-）,女,硕士研究生,从事疟疾分子流行病学研究。E-mail： 1663082396@qq.com
基金资助:
江苏省公益类科研院所自主科研项目(BM2018020);江苏省“科教强卫工程”项目(BM2018020)

Research advance and application of whole-genome sequencing of Plasmodium

LIU Hong¹^,²(), LIU Yao-bao², CAO Jun¹^,²^,^*()

1 Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China
2 Jiangsu Institute of Parasitic Diseases, WHO Collaborating Center for Research and Training on Malaria Elimination, Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Wuxi 214064, China

Received:2020-08-18 Revised:2021-01-15 Online:2021-04-30 Published:2021-04-30
Contact: CAO Jun
Supported by:
Jiangsu Provincial Department of Science and Technology(BM2018020);Jiangsu Provincial Project of Invigorating Health Care through Science, Technology and Education(BM2018020)

摘要/Abstract

摘要：

疟疾是由疟原虫感染引起的一种传染病,是全球最重要的公共卫生问题之一。随着人体疟原虫全基因组测序的完成和测序技术的发展,全基因组测序在疟原虫遗传进化、流行特征、抗药性监测、基因溯源等方面的应用越来越广泛,本文将全基因组测序技术在疟原虫中的研究和应用进展进行综述。

关键词: 疟疾, 疟原虫, 全基因组测序

Abstract:

Malaria is an infectious disease caused by Plasmodium infection and one of the most important public health problems in the world. With the completion of human malaria parasite genome sequence and the development of sequencing technology, the whole-genome sequencing is becoming increasingly useful in determining parasite evolution, revealing epidemic characteristics, drug resistance surveillance, and genetic tracking. This paper reviews the advances in research on and application of the whole-genome sequencing technology for studies on Plasmodium.

Key words: Malaria, Plasmodium, Whole-genome sequencing

中图分类号:

R382.31

刘宏, 刘耀宝, 曹俊. 疟原虫全基因组测序研究进展及其应用[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(2): 265-270.

LIU Hong, LIU Yao-bao, CAO Jun. Research advance and application of whole-genome sequencing of Plasmodium[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2021, 39(2): 265-270.

图/表 1

参考文献 66

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疟原虫种	基因组大小/Mb	染色体数量	AT含量/%	Scaffolds数	基因总数^[11]	蛋白质编码基因数^[11]
恶性疟原虫^[10]	23.3	14	80.7	14	5 712	5 460
间日疟原虫^[7]	29.1	14	60.3	374	6 830	6 677
三日疟原虫^[9]	33.6	14	75.3	63	6 709	6 573
卵形疟原虫柯氏亚种^[9,10]	33.5	14	71.6	4 025	7 280	7 162
卵形疟原虫沃氏亚种^[9,10]	33.5	14	71.1	1 914	8 582	8 421
诺氏疟原虫^[8]	24.4	14	61.3	28	5 483	5 323

疟原虫全基因组测序研究进展及其应用

Research advance and application of whole-genome sequencing of Plasmodium

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