中国寄生虫学与寄生虫病杂志 ›› 2000, Vol. 18 ›› Issue (4): 10-228.

• 论著 • 上一篇    下一篇

两种ELISA方法检测日本血吸虫病循环抗原的比较

裘丽姝;张永红;李浩;薛海筹
  

  1. 中国预防医学科学院寄生虫病研究所!上海200025
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-08-30 发布日期:2000-08-30

COMPARISON OF CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS JAPONICA PATIENTS USING TWO METHODS OF ELISA 

QIU Li-zhu ZHANG Yong-hong LI Hao XUE Hai-chou   

  1. Institute of Parasitic Diseases**;Chinese Academy of Preventive Medicine;Shanghai 200025
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-08-30 Published:2000-08-30

摘要:   [目的 ]比较两种ELISA方法检测血吸虫病患者血清中的循环抗原的效果。 [方法 ]以单克隆抗体为基础的斑点 ELISA和夹心 ELISA。 [结果和结论 ]斑点 ELISA与夹心 ELISA的阳性检出率分别为 48 0 %~ 98 8%和 12 0 %~ 93 8%。两法的特异性依次为 95 4%~ 10 0 %及 90 %。

关键词: 日本血吸虫, 循环抗原, 斑点ELISA, 夹心ELISA

Abstract:  Objective] To compare 2 kinds of ELISA methods for detecting circulating antigen in schistosomiasis patients. [Methods] Monoclonal antibody-based dot-ELISA and sandwich ELISA. [Results and Conclusion] The positive rates of dot-ELISA were higher than or similar to those of sandwich ELISA,the former being 48 0%~98 8% and the latter being 12 0%~93 8%. The specificities were 95 4%~100% and 90 0%, respectively.

Key words: schistosomia japonicum, circulating antigen, dot-ELISA, sandwich-ELISA