环介导等温扩增技术在食源性寄生虫检测中的研究进展

doi:10.12140/j.issn.1000-7423.2026.04.020

中国寄生虫学与寄生虫病杂志 ›› 2026, Vol. 44 ›› Issue (4): 595-599.doi: 10.12140/j.issn.1000-7423.2026.04.020

环介导等温扩增技术在食源性寄生虫检测中的研究进展

董亚如¹()(), 王磊², 吴孜洋¹, 魏紫妍¹, 张子扬¹, 王秋悦¹^,^*()()

¹ 河北科技师范学院动物科技学院/河北省预防兽医重点实验室，河北秦皇岛 066000
² 唐山市食品药品综合检验检测中心，河北唐山 063000

收稿日期:2026-04-07 修回日期:2026-06-02 出版日期:2026-08-30 发布日期:2026-08-12
通讯作者: * 王秋悦（ORCID：0000-0002-5447-0548），女，博士，教授，从事寄生虫病学方面研究。E-mail：13933696970@163.com
作者简介:董亚如（ORCID：0009-0006-5250-6793），女，硕士研究生，从事人兽共患寄生虫病的研究。E-mail：1332499057@qq.com
作者贡献
董亚如负责文献的收集、文章撰写，王磊、吴孜洋、魏紫妍、张子扬、王秋悦负责文章修改。
基金资助:
河北省杰出人才项目;唐山市食品药品综合检验检测中心委托项目(2025HT524)

Research progress on the application of loop mediated isothermal amplification technology in the detection of foodborne parasites

DONG Yaru¹()(), WANG Lei², WU Ziyang¹, WEI Ziyan¹, ZHANG Ziyang¹, WANG Qiuyue¹^,^*()()

¹ Key Laboratory of Preventive Veterinary Medicine in Hebei Province, College of Animal Science & Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066000, Hebei, China
² Tangshan Food and Drug Comprehensive Testing Center, Tangshan 063000, Hebei, China

Received:2026-04-07 Revised:2026-06-02 Online:2026-08-30 Published:2026-08-12
Contact: * E-mail: 13933696970@163.com
Supported by:
Outstanding Talents Project of Hebei Province;Project Commissioned by Tangshan Food and Drug Comprehensive Inspection and Testing Center(2025HT524)

摘要/Abstract

摘要：

食源性寄生虫是影响食品安全的重要因素，其导致的食源性寄生虫病严重威胁人类健康。为有效预防食源性寄生虫感染与传播，保护食品与公众饮食安全，行之有效的检测方法必不可少。目前，食源性寄生虫的检测以病原学检测为主，该法耗时费力，且对检测人员专业性和场地要求较高，应用场景存在一定的局限性；环介导等温扩增（LAMP）技术相较于病原学检测方法而言，具有便捷、高效、不过度依赖设备等优势，在细菌、病毒及寄生虫等领域的核酸检测中得到广泛应用。本文综述了LAMP技术检测原理和在常见食源性寄生虫检测中的应用，探讨LAMP技术在食源性寄生虫检测中的发展趋势。

关键词: 食源性寄生虫, 环介导等温扩增技术, 检测, 应用

Abstract:

Foodborne parasites are an important factor affecting food safety, and the resulting foodborne parasitic diseases have become a serious threat to human health. In order to effectively prevent the infections and transmission of foodborne parasites, and to protect food and public dietary safety, effective tools for detection of foodborne parasitic infection are essential. Currently, detection of foodborne parasites primarily depends on pathogenic methods, which suffer problems of time-consuming and labor-intensive procedures, and high requirement of personnel professionalism and detection sites, resulting in limitations in application scenarios. Loop-mediated isothermal amplification (LAMP) is convenient, highly efficient, and has no excessive dependence on equipment in relative to parasitological techniques, which has been widely used for nucleic acid detection of bacteria, viruses and parasites. This article summarized the principle of LAMP assay and its application in the detection of common foodborne parasites and proposes the development trend of LAMP assay in detection of foodborne parasites.

Key words: Foodborne parasite, Loop-mediated isothermal amplification, Detection, Application

中图分类号:

董亚如, 王磊, 吴孜洋, 魏紫妍, 张子扬, 王秋悦. 环介导等温扩增技术在食源性寄生虫检测中的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2026, 44(4): 595-599.

DONG Yaru, WANG Lei, WU Ziyang, WEI Ziyan, ZHANG Ziyang, WANG Qiuyue. Research progress on the application of loop mediated isothermal amplification technology in the detection of foodborne parasites[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2026, 44(4): 595-599.

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环介导等温扩增技术在食源性寄生虫检测中的研究进展

Research progress on the application of loop mediated isothermal amplification technology in the detection of foodborne parasites

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