中国寄生虫学与寄生虫病杂志 ›› 2018, Vol. 36 ›› Issue (5): 443-449.

• 论著 • 上一篇    下一篇

三种灭螺药物与除草剂复配灭螺效果观察与成本效益分析

陈登1, 朱匡纪2, 张剑锋1, 严晓岚1, 王洪歆2, 杜海娟1, 邵丰尧2, 张晓1, 黄清湘2, 章兰金2, 闻礼永1,*()   

  1. 1 浙江省医学科学院寄生虫病防治研究所,杭州310013
    2 婺城区疾病预防控制中心,金华 321000
  • 收稿日期:2018-02-14 出版日期:2018-10-30 发布日期:2018-11-13
  • 通讯作者: 闻礼永
  • 基金资助:
    国家社会科学基金重大项目(No. 16ZDA237);婺城区科技计划项目(No. 2017-1-34)

Evaluation of the molluscicidal effect and cost of three molluscicides combined with a herbicide

Deng CHEN1, Kuang-ji ZHU2, Jian-feng ZHANG1, Xiao-lan YAN1, Hong-xin WANG2, Hai-juan DU1, Feng-yao SHAO2, Xiao ZHANG1, Qing-xiang HUANG2, Lan-jin ZHANG2, Li-yong WEN1,*()   

  1. 1 Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
    2 Wucheng District Center for Disease Control and Prevention, Jinhua 321000, China
  • Received:2018-02-14 Online:2018-10-30 Published:2018-11-13
  • Contact: Li-yong WEN
  • Supported by:
    Supported by the Major Projects of the National Social Science Foundation of China (No. 16ZDA237) and Science and Technology Planning Project of Wucheng District (No. 2017-1-34)

摘要:

目的 观察灭螺药物25%杀螺胺悬浮剂(SCN)、26%四聚·杀螺胺悬浮剂(MNSC)、50%氯硝柳胺乙醇胺盐可湿性粉剂(WPN)及其与草甘膦复配药在实验室和现场的灭螺效果分析及应用成本,评价各种灭螺药物及复配药推广应用价值。方法 将SCN、MNSC和草甘膦分别配制成有效剂量为2 μl/L(室内浸杀法)、2 ml/m3(现场浸杀法)和2 ml/m2(室内和现场喷洒法)的药剂,同时将WPN粉剂分别配制成有效剂量为2 mg/L(室内浸杀法)、2 g/m3(现场浸杀法)和2 g/m2(室内和现场喷洒法)的药剂。设SCN、SCN复配草甘膦(1 : 1)、MNSC、MNSC复配草甘膦(1 : 1)、WPN复配草甘膦(1 : 1)5个实验组,设WPN为药物对照组,另设草甘膦对照组和清水对照组。室内浸杀实验中,每组分别投放钉螺30只;现场浸杀实验投放螺袋9袋,每袋30只钉螺。分别于施药后24、48和72 h从每个投放点各取3个螺袋,计算钉螺死亡率。室内喷洒实验中,每组投放钉螺100只,于施药后第1、3和7天计算钉螺死亡率;现场喷洒实验中,每组施药1个有螺沟渠小区(面积约为100 m2),施药后第1、3、7天用棋盘式抽样法每组抽取10框钉螺,计算钉螺死亡率。结合灭螺药物及其复配药用药成本、灭螺人工费、除草人工费等进行成本-效益分析。结果 室内浸杀实验结果显示,除SCN复配草甘膦实验组浸杀24 h钉螺死亡率为96.7%(29/30)外,其余4个实验组浸杀24、48、72 h钉螺死亡率均达100%,实验组与药物对照组差异无统计学意义(P > 0.05)。室内喷洒实验结果显示,除SCN复配草甘膦实验组喷洒后第1天钉螺死亡率为91.0%(91/100),第3、7天钉螺死亡率均≥ 95%,其余4个实验组喷洒后第3、7天钉螺死亡率均达100%,实验组与药物对照组差异无统计学意义(P > 0.05)。现场浸杀实验结果显示,除SCN复配草甘膦实验组外(> 95%),其余4个实验组和药物对照组浸杀24、48、72 h钉螺死亡率均达100%,各组差异无统计学意义(P > 0.05)。现场喷洒实验结果显示,MNSC复配草甘膦实验组喷洒后第1、3、7天钉螺死亡率均为100%,高于药物对照组的91.8%(258/281)、95.4%(206/216)、94.5%(147/155)(P < 0.05),其他4个实验组与药物对照组差异无统计学意义(P > 0.05)。现场灭螺中,使用单种灭螺药需先人工除草再使用原药灭螺,需投工两次,SCN、MNSC和WPN 3种原药每100 m2灭螺的合计成本分别为101.00元、101.80元和103.90元;应用复配药灭螺,每100 m2 SCN复配草甘膦、MNSC复配草甘膦和WPN复配草甘膦的合计成本分别为69.50元、70.30元和72.40元,均低于使用单种相应灭螺药原药的成本。结论 灭螺药物SCN、MNSC和WPN及其与草甘膦复配物均能有效灭螺,但综合考虑灭螺的效果和应用成本,推荐在现场喷洒灭螺中应用剂量为2 g/m2或2 ml/m2的灭螺药物复配草甘膦药。

关键词: 灭螺药, 复配, 杀螺效果, 成本-效益

Abstract:

Objective To observe the molluscicidal effect and the cost of molluscicides: 25% niclosamide ethanolamine salt (SCN), 26% suspension of metaldehyde and niclosamide ethanolamine salt(MNSC), and 50% niclosamide ethanolamine salt (WPN) used in combination with glyphosate, in order to evaluate their application value. Methods The SCN, MNSC and the glyphosate were prepared into effective doses of 2 μl/L (for laboratory immersion test), 2 ml/m3 (for field immersion test) and 2 mL/m2 (for spraying test), and the wettable powder of 50% WPN was prepared into effective doses of 2 mg/L (for laboratory immersion test), 2 g/m3 (for field immersion test) and 2 g/m2 (for laboratory and field spraying test). There were 5 experimental groups in this study: SCN, SCN in combination with glyphosate (1 : 1), MNSC, MNSC in combination with glyphosate (1 : 1), and WPN in combination with glyphosate (1 : 1). WPN was used as a drug control. Glyphosate and water control groups were also set. In the laboratory immersion experiment, 30 snails were deposited in each group, and in the field immersion test, 9 bags of snails were deposited, each containing 30 snails. At 24 h, 48 h and 72 h after drug administration, 3 bags of snails were taken back to calculate the snail death rate. In the laboratory spraying test, 100 snails were deposited in each group and the snail death rate was calculated on days 1, 3 and 7 after drug administration. In the field spraying test, a 100 m2 area of snail-containing ditch was administered with drugs, and on days 1, 3 and 7 after drug administration 10 boxes of snails were selected to calculate the snail death rate. The cost-benefit was analyzed based on the cost of molluscicides, labor cost, and herbicide labor cost. Results In the laboratory immersion experiment, the snail death rate reached 100% in all the experimental groups at 24 h, 48 h and 72 h, except for the SCN in combination with glyphosate group at 24 h, in which the death rate was 96.7% (29/30). There was no significant difference between experimental groups and the drug control group. In the laboratory spraying test, the snail death rate reached 100% in all the experimental groups on days 3 and 7, except for the SCN in combination with glyphosate group on day 1 (91.0%, 91/100) and on days 3 and 7(≥95%) at 24 h. There was no significant difference between experimental groups and the drug control group. In the laboratory immersion experiment, the snail death rate reached 100% in all the experimental groups and the drug control group at 24 h, 48 h and 72 h, except for the SCN in combination with glyphosate group (>95%). There was no significant difference between the groups (P > 0.05). In the field spraying test, the snail death rate reached 100% in MNSC in combination with glyphosate group on days 1, 3 and 7, higher than those in the drug control group (91.8%, 258/281; 95.4%, 206/216; 94.5%, 147/155) (P < 0.05). There was no significant difference between the other 4 experimental groups and the drug control group (P > 0.05). The costs of SCN, MNSC and WPN without glyphosate per 100 m2 were 101.00 Yuan, 101.80 Yuan and 103.90 Yuan due to the need of more labor input, while the costs of SCN compounding form, MNSC compounding form and WPN compounding form per 100 m2 were 69.50 Yuan, 70.30 Yuan and 72.40 Yuan, all less than the former.Conclusion On consideration of molluscicidal effect and the application cost, 2 g/m2 or 2 ml/m2 compound drugs of the three types of molluscicides are recommended in field snail control by the method of spraying.

Key words: Molluscicide, Compound drug, Molluscicidal effect, Cost-benefit

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