注射抗TLR9抗体无法有效阻断小鼠体内的TLR9活性：谈TLR9在细胞中的分布

中国寄生虫学与寄生虫病杂志 ›› 2017, Vol. 35 ›› Issue (5): 485-488.

注射抗TLR9抗体无法有效阻断小鼠体内的TLR9活性：谈TLR9在细胞中的分布

刘晓, 李梦梦, 钱锋^*()

第二军医大学附属长征医院风湿免疫科,上海 200003

收稿日期:2017-06-26 出版日期:2017-10-30 发布日期:2018-01-08
通讯作者: 钱锋

Injection of anti-TLR9 antibody cannot efficiently block TLR9 activities in mice: a brief discussion about TLR9 location in cells

Xiao LIU, Meng-meng LI, Feng QIAN^*()

Department of Rheumatology and Immunology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003

Received:2017-06-26 Online:2017-10-30 Published:2018-01-08
Contact: Feng QIAN

摘要/Abstract

摘要：

本文对本刊2017年第35卷第1期上发表的一篇题名为《约氏疟原虫感染小鼠树突状细胞对Th17分化和功能的调控作用》的文章所陈述的方法——用抗TLR9抗体来阻断小鼠体内树突细胞功能,提出异议,因为已有大量的研究工作显示,TLR9总体上是一个细胞内受体,注射抗TLR9抗体无法有效阻断体内TLR9活性。

关键词: TLR9, 亚细胞定位, 抗TLR9抗体, 拮抗剂

Abstract:

In a paper published in this journal (No.1 Vol. 35, 2017) entitled The regulatory effect of dendritic cells on Th17 cell differentiation and function in mouse infected with Plasmodium yoelii, the authors stated in an experimental method that they used anti-TLR9 antibody to block dendritic cell function in mice. We do not think this method works out, because there have been accumulating data revealing that TLR9 is mainly an intracellular receptor, so injection of anti-TLR9 antibody cannot efficiently block TLR9 activities in vivo.

Key words: TLR9, Subcellular location, Anti-TLR9 antibody, Antagonist

中图分类号:

R392.1

刘晓, 李梦梦, 钱锋. 注射抗TLR9抗体无法有效阻断小鼠体内的TLR9活性：谈TLR9在细胞中的分布[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(5): 485-488.

Xiao LIU, Meng-meng LI, Feng QIAN. Injection of anti-TLR9 antibody cannot efficiently block TLR9 activities in mice: a brief discussion about TLR9 location in cells[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2017, 35(5): 485-488.

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