中国寄生虫学与寄生虫病杂志 ›› 2008, Vol. 26 ›› Issue (5): 19-398.

• 研究简报 • 上一篇    下一篇

改良吉氏染色法观察体外培养的阴道毛滴虫分裂繁殖

方正明*,邓伟文,朱红刚,杨振德,刘建军   

  1. 华中科技大学同济医学院病原生物学系,武汉 430030
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-10-31 发布日期:2008-10-31
  • 通讯作者: 方正明

Observation on Mitosis of Trichomonas vaginalis Cultivated in vitro Using Modified Giemsa Staining

FANG Zheng-ming*,DENG Wei-wen,ZHU Hong-gang,YANG Zhen-de,LIU Jian-jun   

  1. Department of Parasitology and Microbiology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-31 Published:2008-10-31
  • Contact: FANG Zheng-ming

摘要:

采用改良吉氏染色法观察体外培养的阴道毛滴虫分裂相。结果显示,分裂间期为66.5%,二分裂期为24.1%,多分裂期为9.4%。二分裂虫体可划分为前有丝分裂期、前期、中期、后期和末期。多分裂虫体可见含3~7或8个子体,其中含3和4个子体的分别占69.1%和24.5%。还可见一些形态异常虫体。

关键词: 阴道毛滴虫, 分裂, 吉氏染色法

Abstract:

The observation showed that the percentage of Trichomonas vaginalis trophozoites at the stages of interphase,binary fission and multiple fission was 66.5%,24.1% and 9.4% respectively. Cells in binary fission could be classified as premitosis phase,prophase,metaphase,anaphase and telophase. 3 to 8 microcosms were seen in one trophozoite under multiple fission and the percentage of trophozoites with 3 and 4 microcosms occupied 69% and 24.5% respectively. Cells with abnormal morphs were also observed.

Key words: Trichomonas vaginalis, Mitosis, Giemsa staining