中国寄生虫学与寄生虫病杂志 ›› 2008, Vol. 26 ›› Issue (3): 10-204.

• 实验研究 • 上一篇    下一篇

福寿螺体内广州管圆线虫Ⅲ期幼虫的形态学观察

张超威,周晓农,吕山,张仪,刘和香
  

  1. 中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫病、丝虫病合作中心,卫生部寄生虫病原与媒介生物学重点实验室,上海 200025
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-06-30 发布日期:2008-06-30

Morphology of Ⅲ Stage Larvae of Angiostrongylus cantonensis in Pomacea canaliculata

ZHANG Chao-wei,ZHOU Xiao-nong,LV Shan,ZHANG Yi,LIU He-xiang
  

  1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-06-30 Published:2008-06-30

摘要: 目的 观察福寿螺体内广州管圆线虫Ⅲ期幼虫的形态学特征。 方法 实验室培养的广州管圆线虫Ⅰ期幼虫感染禁食24 h的福寿螺,61 d解剖,取其肺囊和足肌,常规制作石蜡切片,观察其Ⅲ期幼虫外部形态和内部结构。 结果 Ⅲ期幼虫虫体卷曲,头部圆钝,咽管始于头部顶端的口孔,在咽肠连接处与肠管连接,尾部尖,肛管清晰。幼虫皮层为伊红染色,皮层外有一层无色透明的鞘膜。部分虫体尾部出现圆柱体,有些幼虫体内出现亚腹腺、很短的双管子宫等Ⅳ期幼虫早期特征。 结论 广州管圆线虫Ⅲ期幼虫的形态学特征清晰,对预防广州管圆线虫病流行有一定意义。

关键词: 福寿螺, 广州管圆线虫, Ⅲ期幼虫, 形态学

Abstract: Objective To observe the morphologic characteristics of Ⅲ stage larvae of Angiostrongylus cantonensis from Pomacea canaliculata. Methods P. canaliculata, the intermediate host snail of A. cantonensis, was infected with Ⅰstage larvae of A. cantonensis in laboratory. After 61 days, Ⅲ stage larvae of A. cantonensis were harvested from snail′s lungs and muscle of head-foot, followed by HE stain to observe morphological characteristics. Results The whole body of Ⅲ stage larva was curling with obtuse head. Its pharyngeal canal extends from the buccal hole on the top of the head to the intestines at the pharyngeal intestine joint place, with apex cauda and clear anal tube. The tegument of the Ⅲ stage larva was eosin-stained, with a transparent sheath outside of tegument. Some of the larvae cauda showed in circular cylinder, and some larvae presented ventral gland with two very short uterines which used to be the feature only showed in early Ⅳ stage larva. Conclusion Morphologicall characteristics of the Ⅲ stage larvae is helpful to better understand the life-cycle and the control of A. cantonensis.

Key words: Pomacea canaliculata, Angiostrongylus cantonensis, Ⅲ stage larvae, Morphology