中国寄生虫学与寄生虫病杂志 ›› 2008, Vol. 26 ›› Issue (2): 17-153.

• 研究简报 • 上一篇    下一篇

日本血吸虫线粒体nad4基因部分序列的多态性研究

刘伟1,2,刘毅1,林瑞庆2,戴荣四1,程天印1,朱兴全2 *   

  1. 1湖南农业大学动物医学院, 长沙 410128;2华南农业大学兽医学院, 广州 510642
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-04-30 发布日期:2008-04-30
  • 通讯作者: 朱兴全

Polymorphism in the Partial Mitochondrial nad4 Gene of Schistosoma japonicum

LIU Wei1,2,LIU Yi1,LIN Rui-qing2,DAI Rong-si1,CHENG Tian-yin1,ZHU Xing-quan2 *   

  1. 1 College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;2 College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-04-30 Published:2008-04-30

摘要: 采用PCR技术及DNA序列分析技术对采自湖南省4个不同地区的日本血吸虫的线粒体NADH脱氢酶基因亚基Ⅳ(nad4)部分片段(pnad4)进行克隆及序列分析,获得480 bp的pnad4序列。与GenBank上的日本血吸虫相应基因序列进行比对,发现pnad4序列有一定的种内差异(0.4%~1.3%)。

关键词: 日本血吸虫, 线粒体NADH脱氢酶4, 序列分析

Abstract: The partial NADH dehydrogenase subunit 4 (pnad4) fragment was amplified by polymerase chain reaction (PCR) from Schistosoma japonicum collected from 4 areas in Hunan province, and the amplicons were cloned and sequenced. A 480 bp sequence was obtained and the nucleotide difference between samples from the four locations were 0.4%-1.3%. Low level of sequence variability between and within different populations of S.japonicum was detected. The results are worthwhile for further study on the population genetic structures of S.japonicum.

Key words: Schistosoma japonicum, NADH dehydrogenase subunit 4, Sequence analysis