中国寄生虫学与寄生虫病杂志 ›› 2008, Vol. 26 ›› Issue (2): 16-151.

• 研究简报 • 上一篇    下一篇

日本血吸虫染色体核型分析及C带和G带的制备

黄利红,诸葛洪祥*,周霞,陆祥彬,陈明中,张慧琴   

  1. 苏州大学基础医学系病原生物学教研室, 苏州 215123
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-04-30 发布日期:2008-04-30
  • 通讯作者: 诸葛洪祥

Analysis of Karyotype and Preparation of C-band and G-band in Schistosoma japonicum

HUANG Li-hong,ZHUGE Hong-xiang*,ZHOU Xia,LU Xiang-bin,CHEN Ming-zhong,ZHANG Hui-qin   

  1. Department of Microbiology and Parasitology, Medical College of Soochow University, Suzhou 215123, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-04-30 Published:2008-04-30
  • Contact: ZHUGE Hong-xiang

摘要: 收集日本血吸虫成虫,常规气干法制备血吸虫染色体,改进的BSG法制备C带,胰酶消化法制备G带,分析其染色体核型和C带、G带带型特征。研究表明其核型公式为2n= 4m+6Sm+4St+2性染色体,C带带型公式为2n= 5CI++4CI++3CI+2CT+2CT+,各对染色体显示出特征性的G带。

关键词: 日本血吸虫, 染色体, 核型, C带, G带

Abstract: Chromosomes of Schistosoma japonicum were prepared by usual air drying method, C-band and G-band were made by modified BSG method and enzyme digestion method respectively. Results showed that the karyotype of S. japonicun was 4m+6Sm+4St+2sex chromosome and the C-band formula was 2n= 5CI++4CI++3CI+2CT+2CT+.

Key words: Schistosoma japonicum, Chromosome, Karyotype, C-band, G-band