中国寄生虫学与寄生虫病杂志 ›› 2007, Vol. 25 ›› Issue (4): 21-封三.

• 研究简报 • 上一篇    下一篇

犬钩蚴固体培养基滤纸培养法

许正敏1;李智山2;孙莉1;武小樱1;陶永平1;李明华1   

  1. 1 湖北襄樊职业技术学院医学院病原生物学与免疫学教研室,襄樊 441021; 2 华中科技大学同济医学院附属医院襄樊中心医院,襄樊 441021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-30 发布日期:2007-08-30
  • 通讯作者: 许正敏

Solid Medium-Filter Paper Cultivation of Ancylostoma caninum Larvae

XU Zheng-min1;LI Zhi-shan2;SUN Li1;WU Xiao-ying1;TAO Yong-ping1;LI Ming-hua1   

  1. 1 Department of Pathogenic Biology and Immunology,Medical School,Xiangfan Vocational and Technical College,Xiangfan 441021,China; 2 Xiangfan Central Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Xiangfan 441021,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-30 Published:2007-08-30

摘要: 【提要】 作者采用固体培养基滤纸培养法培养的犬钩蚴检出率高,操作简便、快速,优于传统的试管滤纸培养法。固体培养基由牛肉膏(3 g),蛋白胨(10 g),氯化钠(5 g),琼脂(20 g) 及蒸馏水配制。该法可用于快速诊断钩虫病。

关键词: 犬钩口线虫, 体外培养, 固体培养基, 滤纸

Abstract: 【Abstract】 Consisted of beef extract(3 grams),peptone(10 grams),sodium chloride(5 grams),agar(20 grams) and distilled water,the solid medium was used with filter paper for cultivation of the larvae of Ancylostoma caninum. The method shows higher detection rate and simpler operation than the traditional test tube-filter paper culture technique.

Key words: Ancylostoma caninum, In vitro culture, Solid medium, Filter paper