关键词: 周期型马来丝虫, 副肌球蛋白, 真核表达质粒, COS细胞

Abstract: 【Abstract】 Total RNA was extracted from periodic Brugia malayi. Specific primers were designed on the basis of known sequences of paramyosin gene from B.malayi （BmPmy）. The desired gene was amplified by PCR technique from cDNA. The PCR products were purified and cloned into plasmid pGEM-T by T-A cloning method， transformed into Escherichia coli （E.coli） strain DH5α. The recombinant plasmids were screened and identified by digestion with restriction enzyme and PCR amplification. The right gene fragments encoding BmPmy in positive clones for prokaryotic and eukaryotic expression plasmids were digested with restrictive endonuclease， and were subcloned into pcDNA3.1（+）. The recombinant eukaryotic plasmid （pcDNA3.1-BmPmy） was then transfected into COS-7 cells. The transient expression of BmPmy was examined with RT-PCR. BmPmy mRNA was highly expressed in transfected COS-7 cells.

Key words: Periodic Brugia malayi, Paramyosin, Eukaryotic expressing vector, COS cells