感染卡氏肺孢子虫大鼠血清中可溶性细胞黏附分子-1（sICAM-1）变化的研究

中国寄生虫学与寄生虫病杂志 ›› 2007, Vol. 25 ›› Issue (1): 6-31.

感染卡氏肺孢子虫大鼠血清中可溶性细胞黏附分子-1（sICAM-1）变化的研究

刘克芹1;尹卫东2;薛贵平2;张进顺3

1 河北北方学院附属第一医院检验科，张家口 075000； 2 河北北方学院临床检验教研室，张家口 075000; 3 河北北方学院寄生虫学教研室，张家口 075000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-28 发布日期:2007-02-28
通讯作者: 张进顺

Study on the Changes of Soluble Intercellular Adhesion Molecule-1 Level in Sera of Rats Infected with Pneumocystis carinii

LIU Ke-qin1;YIN Wei-dong2;XUE Gui-ping2;ZHANG Jin-shun3

1 Department of Clinical Laboratory Medicine, The First Affiliated Hospital, Hebei North University, Zhangjiakou 075000, China; 2 Division of Clinical Laboratory Science, Hebei North University, Zhangji-akou 075000, China; 3 Department of Parasitology, Hebei North University, Zhangjiakou 075000, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-28 Published:2007-02-28
Contact: ZHANG Jin-shun

摘要/Abstract

摘要： 目的检测卡氏肺孢子虫肺炎(PCP)大鼠血清中可溶性细胞黏附分子-1（sICAM-1）水平, 以及经复方磺胺甲噁唑（TMP-SMZ）治疗后对sICAM-1含量的影响。方法纯系Wistar大鼠50只, 随机分为 PCP模型组（P组18只）、TMP?鄄SMZ治疗组（S组18只）及正常对照组（N组14只）。P组及S组于大鼠后腿肌肉注射地塞米松（1 mg/只，每周2次、间隔3 d），诱导建立PCP大鼠模型。N组同法注射生理盐水。镜检确认PCP诱导成功后，S组从第3周起灌胃TMP-SMZ[每天1次, 250 mg/（kg·d）］，5 d为1 疗程，间隔2周，共3个疗程。分别于第0、3、6、9及12周取血, 检测血清中sICAM-1水平，观察肺脏、肝脏病理及病原学变化。结果血清sICAM-1水平，P组第3周的(1.847±0.50)ng/ml显著低于第0周的（2.407±0.81) ng/ml(P<0.05)； S组第3周的(1.787±0.59) ng/ml显著低于第0周的（2.478±0.59) ng/ml(P<0.01), 以后逐渐上升。P组第9周的（3.233±0.83) ng/ml、 12周（3.984±0.87) ng/ml显著高于第0周的（2.407±0.81) ng/ml(分别为P<0.05, P<0.01)； S组第12周的（3.621±1.62) ng/ml显著高于第0周的（2.478±0.59) ng/ml(P<0.05)。P组与S组第9、12周[分别为(2.697±0.78) ng/ml及(3.621±1.62) ng/ml] 均显著高于N组(分别为P<0.05、P<0.01)。S组与P组间差异无统计学意义(P>0.05)。结论大鼠血清中sICAM-1含量较低，但在诱导PCP后其含量显著增高。

关键词: 卡氏肺孢子虫, 大鼠, 可溶性细胞黏附分子-1, 组织病理学

Abstract: Objectives To examine sICAM-1 in Pneumocystis carinii pneumonia (PCP) and the effect of TMP-SMZ therapy on its level and on pathological and immunological changes in rats. Methods 50 female Wistar rats were randomly divided into normal group (N group), PCP model group (PCP group) and TMP-SMZ therapy group (SMZ group). 1 mg of dexamethasone was injected intramuscularly twice a week for rats in PCP and SMZ groups to induce PCP. Normal saline was injected for N group in the same way. When the infection was confirmed, TMP-SMZ was given to rats in SMZ group by 25 mg/(kg.d) for 5 days for 3 courses with an interval of 2 weeks. sICAM-1 in serum was detected by ELISA, and the pathological changes in lungs and liver and the Pc in alveoli of lungs were observed. Results The level of sICAM-1 in PCP and SMZ groups at the 3rd week [(1.847±0.50) ng/ml，(1.787±0.59) ng/ml] was lower notably than that at 0 week[（2.407±0.81) ng/ml， [(2.478±0.59) ng/ml respectively](P<0.05), and then increased gradually. It was significantly higher in PCP group at 9th week[（3.233±0.83) ng/ml] and at 12th week[（3.984±0.87) ng/ml] than that of 0 week (P<0.05). Its level in SMZ group at 12th week[（3.621±1.62) ng/ml] was also higher than that in 0 week [（2.478±0.59) ng/ml] (P<0.05). sICAM-1 level in both PCP and SMZ groups at 9th week and 12th week was higher than that of N group (P<0.05，P<0.01). There was no significant difference between SMZ and PCP groups at 9th week and 12th week (P>0.05). Conclusion The sICAM-1 level in rats was low but significantly increases after the induction of PCP.

Key words: Pneumocystis carinii, Rat, Soluble intercellular adhesion molecule-1（sICAM-1）, Histopathology

刘克芹;尹卫东;薛贵平;张进顺. 感染卡氏肺孢子虫大鼠血清中可溶性细胞黏附分子-1（sICAM-1）变化的研究[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(1): 6-31.

LIUKe-qin;YINWei-dong;XUEGui-ping;ZHANGJin-shun. Study on the Changes of Soluble Intercellular Adhesion Molecule-1 Level in Sera of Rats Infected with Pneumocystis carinii[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2007, 25(1): 6-31.

[1]	马志强, 王霖, 李生浩, 徐晶晶, 李才信, 刘媛, 张燕玲, 束秋红, 庄杉杉, 何姝美琪, 王文林, 王卫群. 丰宫并殖吸虫感染大鼠后诱导肺组织细胞自噬的初探[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(1): 35-42.
[2]	刘静, 辛致炜, 裴培, 傅晓茵, 廖德君, 卢韵宇, 薛飒, 廖振捷, 刘登宇. 人芽囊原虫感染大鼠的方式改良及病理切片观察[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(2): 161-167.
[3]	任宾，樊海宁*，邓勇，王海久，任利. 泡球蚴囊液对大鼠肝星状细胞MAPK信号通路5个相关基因的影响[J]. 中国寄生虫学与寄生虫病杂志, 2015, 33(2): 8-114-117,121.
[4]	周永华, 范红结, 张英, 黄玉政, 许永良, 陶永辉, 高琪. 慢性弓形虫感染大鼠海马蛋白质组的研究[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(6): 8-454-457.
[5]	安然，黄谋，张秦，郑宏*. 细粒棘球蚴感染大鼠过敏反应中IL-10水平的初步研究[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(2): 17-86-88.
[6]	闻晓波，冉旭华*，王春仁，宋佰芬，魏晓曼，李晓娟，王密，苗艳. 肝片形吸虫重组谷胱甘肽?鄄S?鄄转移酶对 SD大鼠的免疫原性分析[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(1): 10-46-48,53.
[7]	包永星，张月芬，倪雅琼，谢增如，齐洪志，毛睿，杨玉刚，温浩*. 电子线治疗大鼠继发性泡球蚴病的实验研究[J]. 中国寄生虫学与寄生虫病杂志, 2011, 29(2): 9-127-129.
[8]	薛剑;徐莉莉;强慧琴;张永年;肖树华. 用三苯双脒、吡喹酮和青蒿琥酯临床给药方案治疗感染华支睾吸虫大鼠的研究[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(3): 2-171.
[9]	陈金铃;段义农;王建新;朱丹丹;秦永伟. 卡氏肺孢子虫p55抗原片段免疫原性的研究[J]. 中国寄生虫学与寄生虫病杂志, 2009, 27(6): 9-497.
[10]	周泠;周必英. 黄芩苷对卡氏肺孢子虫肺炎大鼠TNF-α和sICAM-1的调节作用[J]. 中国寄生虫学与寄生虫病杂志, 2009, 27(2): 11-147.
[11]	孟晓丽;殷国荣;刘红丽;王海龙. RH株弓形虫速殖子体外入侵大鼠肠上皮细胞与增殖的动态观察[J]. 中国寄生虫学与寄生虫病杂志, 2009, 27(1): 9-42.
[12]	肖树华;薛剑;MarcelTANNER;张永年;JenniferKEISER;JürgUTZINGER;强慧琴;刘晓云. 三苯双脒、青蒿琥酯、蒿甲醚和吡喹酮单剂、多剂或联合用药治疗大鼠华支睾吸虫感染的实验研究[J]. 中国寄生虫学与寄生虫病杂志, 2008, 26(5): 1-326.
[13]	李林杰;包怀恩;潘卫. 应用双向电泳及蛋白质印迹分析弓形虫特异性抗原[J]. 中国寄生虫学与寄生虫病杂志, 2008, 26(4): 17-320.
[14]	戴晓冬;秦元华;周长海;郑莉莉;邵伟军;陶琳;崔昱. 补骨脂与鸦胆子对感染卡氏肺孢子虫大鼠IL-2水平和NK细胞活性的影响[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(5): 21-438.
[15]	王建新;;段义农;陈金铃;董永生. 卡氏肺孢子虫p55抗原基因片段原核表达质粒的构建与表达[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(4): 13-332.