中国寄生虫学与寄生虫病杂志 ›› 2006, Vol. 24 ›› Issue (3): 22-203.

• 研究简报 • 上一篇    下一篇

元江县微小按蚊卵巢营养细胞多线性染色体观察

石焕焕;黄颉刚;田春林;袁志刚   

  1. 广西医科大学寄生虫学教研室, 南宁 530021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-06-30 发布日期:2006-06-30

Observation on Polytene Chromosomes of the Ovarian NurseCell of Anopheles minimus from Yuanjiang

SHI Huan?-huan;HUANG Jie-gang;TIAN Chun-lin;YUAN Zhi-gang   

  1. Department of Parasitology, Guangxi Medical University, Nanning 530021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-06-30 Published:2006-06-30

摘要: 云南省元江县采集的微小按蚊分别单只雌蚊驯养,解剖分离卵巢营养细胞,染色后光镜观察、记录染色体图谱。经观察365个卵巢多线染色体标本。其染色体由5臂共3条染色体组成:Ⅰ号染色体为具端着丝点的性染色体(X染色体),仅见一臂;Ⅱ号为1对具亚中着丝点的常染色体,分右臂(2R)和左臂(2L);Ⅲ号为1对具中着丝点的常染色体,含右臂(3R)和左臂(3L)。与广西微小按蚊卵巢营养细胞多线染色体比较,发现有12处差异。

关键词: 微小按蚊, 卵巢营养细胞, 多线染色体

Abstract: Anopheles minimus collected from Yuanjiang, Yunnan Province, were bred with standard methods in lab. The ovarian nurse cells of A.minimus were separated and stained, and the whole polytene chromosomes were photographed under light microscope and compared with A.minimus from Guangxi. 365 samples of ovarian nurse cells were observed. The chromosomes included one telocentric sex-chromosome X, two submetacentric autosomes II(autosome II right arm, 2R and autosome II left arm, 2L) and two metacentric autosomes III(autosome III right arm, 3R, and autosome III left arm, 3L). The X is the shortest chromosome and the 2R is the longest one. In comparison with the pattern of polytene chromosomes of A. minimus from Guangxi, difference at 12 positions has been found at the parts of arms in banding sequences.

Key words: Anopheles minimus, Ovarian nurse cells, Polytene chromosome