CpG DNA重组质粒对猪囊尾蚴抗原的免疫佐剂效应研究

中国寄生虫学与寄生虫病杂志 ›› 2006, Vol. 24 ›› Issue (2): 19-152.

CpG DNA重组质粒对猪囊尾蚴抗原的免疫佐剂效应研究

景志忠;蒙学莲;王佩雅;窦永喜;李辉;骆学农;郑亚东;才学鹏

中国农业科学院兰州兽医研究所，甘肃省动物寄生虫病重点实验室，家畜疫病病原生物学国家重点实验室，兰州 730046

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-04-30 发布日期:2006-04-30

Adjuvant Effect of CpG DNA Recombinant Plasmid onAntigen of Cysticercus cellulosae

JING Zhi-zhong;MENG Xue-lian;WANG Pei-ya;DOU Yong-xi;LI Hui;LUO Xue-nong;ZHENG Ya-dong;CAI Xue-peng

Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences；Gansu Provincial Key Laboratory of Animal Parasitology, Lanzhou 730046, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-04-30 Published:2006-04-30

摘要/Abstract

摘要： 用CpG基序寡核苷酸的重组质粒（CpG DNA）与猪囊尾蚴抗原制备疫苗免疫小鼠，检测小鼠抗囊尾蚴总抗体和IgG2a抗体亚类以及体外诱导免疫脾细胞分泌白介素-4（IL-4）和干扰素-γ（IFN-γ）的含量，观察其免疫佐剂效应。发现CpG DNA重组质粒中无论插入CpG基序多或少都能增强小鼠抗囊尾蚴总抗体和IgG2a抗体亚类含量，都具有免疫佐剂效应，但其免疫强度有差别，其中CpG2的佐剂效应最强；此外，CpG DNA重组质粒能与铝胶、206佐剂配伍发挥免疫增强协同作用。

关键词: CpG DNA, 佐剂效应, Th1型, 协同作用

Abstract: In order to prove the adjuvant effect of CpG DNA recombinant plasmid, the total antibodies and their IgG2a subtype induced by antigen of Cysticercus cellulosae, and content of IL-4 and IFN-γ secreted from splenic cell of mouse immunized were measured. The recombinant plasmids showed an adjuvant effect, and CpG2 was the best adjuvant among the plasmids. It is proved that the CpG DNA possesses a synergistic effect with Al(OH)3 and 206 adjuvant, and is an effective Th1 type adjuvant in mice.

Key words: CpG DNA, Adjuvant effect, Th1 type, Synergistic effect

景志忠;蒙学莲;王佩雅;窦永喜;李辉;骆学农;郑亚东;才学鹏. CpG DNA重组质粒对猪囊尾蚴抗原的免疫佐剂效应研究[J]. 中国寄生虫学与寄生虫病杂志, 2006, 24(2): 19-152.

JINGZhi-zhong;MENGXue-lian;WANGPei-ya;DOUYong-xi;LIHui;LUOXue-nong;ZHENGYa-dong;CAIXue-peng. Adjuvant Effect of CpG DNA Recombinant Plasmid onAntigen of Cysticercus cellulosae[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2006, 24(2): 19-152.