应用基因芯片分析HLA-DRB与晚期肝脾型日本血吸虫病的相关性

中国寄生虫学与寄生虫病杂志 ›› 2005, Vol. 23 ›› Issue (6): 3-395.

应用基因芯片分析HLA-DRB与晚期肝脾型日本血吸虫病的相关性

程喻力^1,3,徐明星²,宋文剑³,杨燕²,刘文琪^1*,李雍龙¹,裘敏燕⁴,吴海⁴

1 华中科技大学同济医学院寄生虫教研室,武汉 430030； 2 武汉市疾病预防控制中心血吸虫病研究所,武汉 430022；3 江汉大学医学与生命科学学院病原生物学教研室,武汉 430056； 4 上海博星基因芯片有限公司,上海 200092

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-12-30 发布日期:2005-12-30

Microarray DNA Chip in Analyzing the Association BetweenHLA-DRB and Advanced Hepatosplenic Schistosomiasis

CHENG Yu-li,XU Ming-xing,SONG Wen-jian,YANG Yan,LIU Wen-qi*,
LI Yong-long,QIU Min-yan,WU Hai

Department of Parasitology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

Received:1900-01-01 Revised:1900-01-01 Online:2005-12-30 Published:2005-12-30

摘要/Abstract

摘要： 目的探讨人类白细胞抗原Ⅱ类(HLA-Ⅱ）基因多态性与晚期肝脾型日本血吸虫病的遗传关联性。方法应用基因芯片分析技术对武汉市蔡甸45例晚期肝脾型日本血吸虫病患者（实验组）和44例慢性日本血吸虫病患者（对照组）的HLA-Ⅱ基因DRB位点等位基因进行基因分型，并比较两组各等位基因频率以及与晚期肝脾型日本血吸虫病的相关性。结果实验组HLA-DRB1*04x等位基因频率明显高于对照组（P＜0.01， RR=3.928），而对照组HLA-DRB1*15x等位基因频率明显高于实验组（P＜0.01， RR=0.050）。等位基因DRB1*15x总与DRB5*010x/020x连锁，对照组DRB1*15x-DRB5*010x/020x连锁体频率明显高于实验组（P＜0.01）。结论 HLA-DRB1*04x与晚期肝脾型日本血吸虫病呈正相关，而HLA-DRB1*15x与晚期血吸虫病呈负相关。

关键词: 肝脾型血吸虫病, 人类白细胞抗原基因DRB位点, 基因芯片

Abstract: Objective To explore possible associations between host polymorphism of HLA classⅡgenotypes and advanced hepatosplenic schistosomiasis japonica. Methods 45 advanced schistosomiasis patients (experimental group） and 44 age-and sex-matched patients with chronic schistosomiasis （control group） from the same area were investigated for their HLA class II gene DRB genotypes by genotyping the alleles using microarray DNA chip. The correlation of allele frequencies to advanced hepatosplenic schistosomiasis was compared for the two groups. Results HLA-DRB1*04x exhibited markedly higher frequency in advanced patients than that in control group （P＜0.01， RR=3.928）. In contrast，the frequency of HLA-DRB1*15x in advanced patients was much lower when compared with that in control group （P＜0.01， RR=0.050）. Besides，the significant allele HLA-DRB1*15x displayed concurrence with allele DRB5*010x/020x. The linked gene haplotype DRB1*15x-DRB5*010x/020x showed significantly higher incidence in control group than in experimental group （P＜0.01）. Conclusion Allele HLA-DRB1*04x is positively，while HLA-DRB1*15x is negatively，correlated with advanced hepatosplenic schistosomiasis.

Key words: Hepatosplenic schistosomiasis, HLA-DRB, DNA chip

程喻力;徐明星;宋文剑;杨燕;刘文琪;李雍龙;裘敏燕;吴海. 应用基因芯片分析HLA-DRB与晚期肝脾型日本血吸虫病的相关性[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(6): 3-395.

CHENGYu-li;XUMing-xing;SONGWen-jian;YANGYan;LIUWen-qi*;LIYong-long;QIUMin-yan;WUHai. Microarray DNA Chip in Analyzing the Association BetweenHLA-DRB and Advanced Hepatosplenic Schistosomiasis[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 23(6): 3-395.

[1]	陈木新，陈家旭*. 基因芯片技术在人体锥虫生物学特性研究方面的进展[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(4): 16-377-381.
[2]	章俊华;刘文琪;李雍龙;龙小纯. Ⅱ类人白细胞抗原等位基因与晚期肝脾型日本血吸虫病的相关性研究[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(1): 2-9.
[3]	田海生;朱昌亮;高晓红;马磊;沈波;李秀兰;吴观陵. 淡色库蚊对溴氰菊酯抗药性和敏感性相关基因克隆和序列分析[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(4): 1-197.