阿米巴活体微培养法

中国寄生虫学与寄生虫病杂志 ›› 2005, Vol. 23 ›› Issue (6): 17-456.

阿米巴活体微培养法

陈洪友,姜庆五,李勤学,李子华

复旦大学公共卫生学院流行病教研室,上海 200032

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-12-30 发布日期:2005-12-30

A Micro-Culture Method for Continuous Observationof Free-living Amoebae

CHEN Hong-you,JIANG Qing-wu,LI Qin-xue,LI Zi-hua

Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China

Received:1900-01-01 Revised:1900-01-01 Online:2005-12-30 Published:2005-12-30

摘要/Abstract

摘要： 目的建立一种既可做阿米巴与病原体共培养实验，又可连续观察阿米巴活体形态细微变化的培养技术。方法以24孔细胞培养板覆盖玻片为容器，37 ℃下微量悬滴将阿米巴与白色念珠菌共同培养, 普通光学显微镜（×1000）下连续观察阿米巴的形态、活动能力、吞噬的细菌等情况。结果经微量悬滴培养在普通光学显微镜油镜下可连续观察阿米巴各种形态，滋养体呈T、K、Y字形态，并可连续观察吞噬白色念珠菌的过程。结论本法可观察到常规固定染色标本无法见到的阿米巴滋养体T、K、Y字形态及其吞噬白色念珠菌过程。

关键词: 自由生活阿米巴, 白色念珠菌, 活体形态观察, 微量悬滴培养

Abstract: Objective To establish a method for co-culture of amoebae and endosymbionts, also for continuously observing the microphenotype of amoebae. Methods 24 wells culture plate with cover glass on the wells was used as containers. Amoebae and Candida albicans were co-cultured in microdrop of medium in the wells at 37℃, and observed under ×1000. Results Continuous observation revealed trophozoites in various shapes like letters T, K, or Y, their movement and ingestion phenomenon were observed. Conclusion The micro-culture method is useful in observing the amoebal morphology and its phagocytic process to Candida albicans.

Key words: Free-living amoeba, Candida albicans, Morphology of living object, Micro-culture

陈洪友;姜庆五;李勤学;李子华. 阿米巴活体微培养法[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(6): 17-456.

CHENHong-you;JIANGQing-wu;LIQin-xue;LIZi-hua. A Micro-Culture Method for Continuous Observationof Free-living Amoebae[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 23(6): 17-456.

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