用mtCO技术测定云南及贵州四地区的牛带绦虫

中国寄生虫学与寄生虫病杂志 ›› 2003, Vol. 21 ›› Issue (1): 7-23.

用mtCO技术测定云南及贵州四地区的牛带绦虫

王正蓉¹,包怀恩²

贵阳医学院临床检验学教研室,贵阳 550004

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-02-28 发布日期:2003-02-28

Identification of Taenia saginata by mtCOⅠ in Four Areas of Yunnan and Guizhou Provinces

WANG Zheng-rong¹,BAO Huai-en²

Department of Laboratory Medicine,Guiyang Medical College,Guiyang 550004

Received:1900-01-01 Revised:1900-01-01 Online:2003-02-28 Published:2003-02-28

摘要/Abstract

摘要： 　　目的　用mtCO技术鉴定云南省大理及贵州省都匀、从江等地所发现的是牛带绦虫或牛带绦虫亚洲亚种。　方法　采集成虫样本,抽提DNA,PCR扩增线粒体细胞色素C氧化酶(mtCO)部分基因片段并测序,经PHYLIP软件包处理,计算遗传距离并构建系统发育树状图。　结果　云南省兰坪、大理和贵州省都匀的标本mtCO基因片段序列与已知牛带绦虫亚洲亚种的序列相同。贵州从江的标本mtCO基因片段序列与已知牛带绦虫的序列相同。两组基因片段的同源性达97.44%,氨基酸序列同源性达99.16%。系统发育树状图显示,牛带绦虫亚洲亚种和牛带绦虫最为接近,远离猪带绦虫及其他绦虫。　结论　云南省兰坪、大理及贵州省都匀存在牛带绦虫亚洲亚种,贵州省从江发现的虫种为牛带绦虫。

关键词: 牛带绦虫, 牛带绦虫亚洲亚种, 线粒体细胞色素C氧化酶

Abstract: 　Objective To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province. Methods Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunitⅠ(mtCOⅠ) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package. Results The mtCOⅠgene sequences of Lanping sample were identical to that of T.saginata asiatica known in Taiwan, and the samples obtained from Dali and Duyun showed the same mtCOⅠgene sequences, while the sample from Congjiang had the same mtCOⅠgene sequences as T.saginata . The homology between these two groups of gene sequences was 97.44%, while the homology of amino acid sequences reached to 99.16%. The constructed phylogenetic tree revealed that the relationship between T.saginata asiatica and T.saginata is closer, both are distant relative to T.solium and other species of cestodes. Conclusion The Taenia prevalent in Lanping, Dali and Duyun is identified as T.saginata asiatica , while that isolated in Congjiang is the typical T.saginata.

Key words: Taenia saginata, Taenia saginata asiatica, mitochondrial cytochrome C oxidaseⅠ

王正蓉;包怀恩. 用mtCO技术测定云南及贵州四地区的牛带绦虫[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(1): 7-23.

WANGZheng-rong;BAOHuai-en. Identification of Taenia saginata by mtCOⅠ in Four Areas of Yunnan and Guizhou Provinces[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2003, 21(1): 7-23.

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