感染细粒棘球蚴绵羊诱发过敏性休克期间IgG、IgG1和IgE水平的探讨

中国寄生虫学与寄生虫病杂志 ›› 2003, Vol. 21 ›› Issue (1): 13-45.

感染细粒棘球蚴绵羊诱发过敏性休克期间IgG、IgG1和IgE水平的探讨

郑宏¹,徐志新¹,杨戈雄²,温浩³

1 新疆医科大学第一附属医院麻醉科,乌鲁木齐 830054 2 武警新疆总队医院麻醉科,乌鲁木齐 830091 3 新疆医科大学第一附属医院普外科,乌鲁木齐 830054

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-02-28 发布日期:2003-02-28

Study on the Level of Specific IgG, IgG1 and IgE During Anaphylactic Shock in Sheep Induced by Echinococcus granulosus

ZHENG Hong¹,XU Zhi-xin^1_,Yang Ge-xiong²,WEN Hao³

1 Department of Anesthesiology,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054, 2 Department of Anesthesiology,Xinjiang Corps Hospital of Chinese People′s Armed Ploice Forces,Urumqi 830054, 3 Department of General Surgery,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054

Received:1900-01-01 Revised:1900-01-01 Online:2003-02-28 Published:2003-02-28

摘要/Abstract

摘要： 　　目的　测定感染细粒棘球蚴(E.g)绵羊诱发过敏性休克期间特异性IgG、IgG1和IgE抗体水平。了解抗原B对人工感染E.g绵羊IgG抗体的反应性。　方法　从绵羊E.g囊液中制备抗原B和E.g囊液粗制抗原,ELISA测定感染E.g绵羊诱发休克期间特异性IgG、IgG1和IgE抗体的动态变化。　结果　感染E.g绵羊6个月,特异性IgG、IgG1和IgE抗体水平较正常绵羊显著升高;诱发休克后特异性IgE抗体水平显著下降,尤其因休克致死的绵羊下降更为明显;IgG及IgG1抗体的衰减时间不同,趋势各不相同;抗原B和E.g囊液粗制抗原与血清IgG抗体反应阳性率分别为91%、32%。　结论　特异性IgE是导致棘球蚴病所致过敏性休克的主要抗体,而IgG和IgG1抗体也起着重要作用。抗原B与感染E.g绵羊IgG抗体的血清反应性较好,可作为一种血清免疫学诊断方法监测绵羊感染E.g的状况。

关键词: 细粒棘球蚴, 绵羊, 过敏性休克, 抗体, 抗原B

Abstract: 　Objective To investigate the change of specific antibodies IgG, IgG1 subclass and IgE in sheep infected with Echinococcus granulosus(E.g) during anaphylactic shock, and to observe antigen B reactivity against IgG antibody in E.g|infected sheep. Methods Antigen B and crude antigen were prepared with E.g cyst fluid (EgCF) from infected sheep. The enzyme|linked immunosorbent assay(ELISA) was used for detecting the level of specific IgG, IgG1 and IgE during anaphylactic shock in sheep induced by E.g. Results The level of specific IgG, IgG1 and IgE was significantly higher in the infected sheep after 6 months than that of the uninfected control group (P<0.01). The IgE level decreased rapidly after anaphylactic shock induced, especially when the sheep was dying. Following an antigen challenge the sheep showed a general decrease in total IgG and IgG1 subclass. The total IgG showed a slight change at the beginning,followed by a decrease 1 h after challenge. The decrease of IgG1 subclass was more significant than the total IgG in 40 min after challenge injection. The positive rate was 91% for antigen B and 32% for crude antigen of EgCF against IgG antibody in E.g|infected sheep. Conclusion The specific IgE plays a major role in the anaphylactic shock, while IgG and IgG1 antibodies are also important. Antigen B derived from sheep E.g cyst fluid appears to be useful in serodiagnosis of and monitoring on the infection status in sheep.

Key words: Echinococcus granulosus, sheep, anaphylactic shock, antibody, Antigen B

郑宏;徐志新;杨戈雄;温浩. 感染细粒棘球蚴绵羊诱发过敏性休克期间IgG、IgG1和IgE水平的探讨[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(1): 13-45.

ZHENGHong;XUZhi-xin_;YangGe-xiong;WENHao. Study on the Level of Specific IgG, IgG1 and IgE During Anaphylactic Shock in Sheep Induced by Echinococcus granulosus[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2003, 21(1): 13-45.

[1]	逯君霞, 许军英, 赵彬, 王芊文, 李文华, 耿玉庆, 侯隽, 吴向未, 陈雪玲. 细粒棘球蚴感染诱导巨噬细胞表达CD73和A2AR抑制炎症反应[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 559-566.
[2]	蒉嫣, 薛垂召, 王旭, 刘白雪, 王莹, 王立英, 杨诗杰, 韩帅, 伍卫平, 肖宁. 2021年全国棘球蚴病防治进展[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 142-148.
[3]	郭刚, 任远, 焦红杰, 武娟, 郭宝平, 齐文静, 李军, 张文宝. 腹腔感染棘球蚴微囊对小鼠肝脏多房棘球蚴感染及致病的影响[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 156-162.
[4]	路伟民, 杨小涛, 朱瑛, 张鸿, 李霁伟, 王艳春. 儿童肺细粒棘球蚴病1例[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 253-256.
[5]	焦红杰, 齐文静, 郭刚, 包建玲, 吴川川, 宋传龙, 李军, 张文宝, 严媚. 细粒棘球蚴抗原B对小鼠巨噬细胞RAW264.7的极化作用[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(1): 23-28.
[6]	邹伟浩, 吴蔚玲, 廖远鹏, 陈敏, 彭鸿娟. 刚地弓形虫抗缓殖子期抗原1单克隆抗体的制备与应用[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 587-593.
[7]	郭璐, 吴小霞, 段兰利, 王冰洁, 徐宁, 阿热艾·阿哈太, 乌云花, 赵莉, 班万里, 陈云英, 余婉蓉, 刘帅, 潘星羽, 吾力江·卡马力, 徐晶, 穆尼拉·特列吾汗, 张壮志. 新疆部分地区牛、羊细粒棘球蚴感染情况调查及基因多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 603-609.
[8]	毋德芳, 付永, 任宾, 张耀刚, 许晓磊, 庞明泉, 樊海宁. 青海人源两型棘球绦虫（棘球蚴）遗传多样性及分化时间研究[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 610-615.
[9]	侯娇, 温浩, 王明坤, 蒋铁民, 房彬彬, 李静, 张传山, 王慧. 肝细粒棘球蚴病手术患者病灶活性状态的影响因素分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(3): 309-314.
[10]	乔世源, 周雪, 刘程豪, 姜慧娇, 卜媛媛, 陈雪玲, 吴向未. 阿苯达唑载药囊泡对细粒棘球蚴原头节活性的影响[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(3): 324-329.
[11]	严积灿, 于文昊, 侯立朝, 张灵强, 许晓磊, 王海久, 卢倩, 樊海宁. 高龄腹腔-皮下细粒棘球蚴病诊疗1例报告[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 132-135.
[12]	朱江, 黄海军, 张望, 梅虎, 朱时雨, 宋思凯, 张军. 合并囊内胆漏的肝细粒棘球蚴病患者手术方式的分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 50-55.
[13]	马文梅, 桑伟, 艾麦提·牙森, 佐力克, 付莉, 苗娜. 核因子-κB/髓样分化分子88在细粒棘球蚴病患者肝纤维化中的作用[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(6): 779-783.
[14]	周文正, 孙俊刚, 赵喜滨, 曹力. 三维适形调强放疗对大鼠继发性股骨细粒棘球蚴感染的疗效研究[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(4): 443-448.
[15]	黄燕, 喻文杰, 尚婧晔, 何伟, 张光葭, 王奇, 杨柳, 廖沙, 李汭芮, 姚人新, 曾明才, 张福斌, 李树成, 刘阳, 钟波, 王谦. 阿苯达唑和吡喹酮交替联合用药治疗藏族牧民棘球蚴病的效果观察[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(2): 171-177.