大鼠卡氏肺孢子虫肺炎的免疫应答

中国寄生虫学与寄生虫病杂志 ›› 2000, Vol. 18 ›› Issue (1): 13-42.

大鼠卡氏肺孢子虫肺炎的免疫应答

刘斌¹;何礼贤²;瞿介明²;胡必杰²;王葆青²;李锡莹²

上海第二医科大学附属仁济医院呼吸科!上海200001(刘斌);上海医科大学附属中山医院肺病学教研室!上海200032(何礼贤;瞿介明;胡必杰;王葆青);

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-02-28 发布日期:2000-02-28

IMMUNE RESPONSE IN PNEUMOCYSTIS CARINII PNEUMONIA IN RATS

LIU Bin¹;HE Li xian ²;QU Jie ming ²;HU Bi jie ²;WANG Bao qing ²;LI Xi ying ²

1 Department of Respiratory;Renji Hospital;Shanghai Second Medical University;Shanghai 200001; 2 Department of Pulmonary Diseases;Zhongshan Hospital;Shanghai Medical University,Shanghai 200032

Received:1900-01-01 Revised:1900-01-01 Online:2000-02-28 Published:2000-02-28

摘要/Abstract

摘要： 　　[目的 ]探讨应用糖皮质激素 (GC)诱发大鼠卡氏肺孢子虫肺炎 (Pneumocystiscariniipneumonia ,PCP)的免疫应答。 [方法 ]SD大鼠皮下注射GC建立免疫抑制PCP模型 ,测定外周血淋巴细胞比例、CD4+ T细胞 /CD8+ T细胞比例 ,肺泡灌洗液 (bronchoalveolarlavagefluid ,BALF)中淋巴细胞比例、sIL 2R和TNF α值。 [结果 ]①免疫抑制后外周血淋巴细胞比例、CD4+ T细胞 /CD8+ T细胞比例降低 ,BALF中淋巴细胞比例、TNF α、sIL 2R均降低 ;②BALF中TNF α、外周血CD4+ T细胞 /CD8+ T细胞比值在PCP组中最低 ;③PCP组的BALF中淋巴细胞比例比PC阴性对照组明显升高。 [结论 ]使用GC造成大鼠免疫抑制 ,诱发PC感染后 ,CD4+ 减少及TNF α分泌降低。

关键词: 糖皮质激素, 免疫抑制, 卡氏肺孢子虫肺炎, 炎性免疫应答

Abstract: 　Objective] To study the inflammatory immune response to Pneumocystis canii pneumonia(PCP) in rats induced by glucocorticoid(GC).[Methods] The model of PCP was set up by injecting GC subcutanously to SD rats. Lymphocyte proportion in peripheral blood, CD4 +/CD8 + T cell ratio of PBL and lymphocyte proportion in the BALF were measured. The levels of sIL 2R and TNF α in the BALF were detected.[Results]① After the rats were immunospressed, the lymphocyte proportion in the peripheral blood and CD4 +/CD8 + ratio of PBL, and the lymphocyte proportion in the BALF were decreased, and the levels of sIL 2R, TNF α in BALF were reduced. ② The lowest levels of TNF α in BALF and CD4 +/CD8 + T cells of PBL were observed in PCP group; ③ The lymphocyte proportion in the BALF was significantly higher in PCP group than in PC negative group.[Conclusion]The reduction in the level of TNF α and CD4 + /CD8 + T cell ratio in rats treated with GC might result in PCP infection under immunosuppressive condition.

Key words: Glucocorticoid, immunosuppression, Pneumocystis carini pneumonia, inflammatory immune response.

刘斌;何礼贤;瞿介明;胡必杰;王葆青;李锡莹. 大鼠卡氏肺孢子虫肺炎的免疫应答[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(1): 13-42.

LIUBin;HELixian;QUJieming;HUBijie;WANGBaoqing;LIXiying. IMMUNE RESPONSE IN PNEUMOCYSTIS CARINII PNEUMONIA IN RATS[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2000, 18(1): 13-42.

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