中国寄生虫学与寄生虫病杂志 ›› 1998, Vol. 16 ›› Issue (5): 357-359.

• 论著 • 上一篇    下一篇

日本血吸虫童虫培养细胞SOD和MDA动态变化的研究

蒋明森; 陈晓蓓**; 董惠芬; 杨明义; 倪永晖; 周述龙   

  1. 湖北医科大学寄生虫学教研室 * * 湖北医科大学附属第一医院传染科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-10-31 发布日期:1998-10-31

STUDIES ON DYNAMIC CHANGES OF SOD AND MDA IN CULTURED CELLS FROM SCHISTOSOMA JAPONICUM SCHISTOSOMULA

Jiang Mingsen; Chen Xiaobei* ; Dong Huifen; Yang Mingyi; Ni Yonghui; Zhou Shulong   

  1. Department of Medical Parasitology and Research Laboratory of Schistosomiasis; Hubei Medical University; Wuhan 430071  *Department of Infectious Disease; The First Affiliated Hospitol of HubeiMedical University ,Wuhan 430060
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-10-31 Published:1998-10-31

摘要: 目的:观察日本血吸虫童虫细胞在体外培养过程中超氧化物歧化酶(SOD)和丙二醛(MDA)含量的动态变化,探讨培养细胞的退化过程。方法:应用邻苯三酚自氧化法及硫代巴比妥酸法分别测定童虫细胞在培养0-54d过程中SOD和MDA含量的变化。结果:在培养第12dSOD出现一个高峰,以后一直处于低平状态。MDA在培养第18d和第48d分别各有一个高峰,其后均逐渐降低。结论:日本血吸虫童虫培养细胞呈逐步退化过程。

关键词: 日本血吸虫, 童虫, 培养细胞, SOD, MDA

Abstract:

AIM:To observe the dynamic changes of superoxide dismutase(SOD) and

maldondialdehyde(MDA) in cultured cells from Schistosoma japonicum schistosomula and to explore

the degeneration process of cultured cells. METHODS: Pyrogallol method and thiobarbituric acid

method were used to determinate the levels of SOD and MDA , respectively,in the cells cultured

for 0-54 days. RESULTS: SOD in the cells reached a peak level on d12 of the early stage of

culture and then decrease. The MDA levels in the cultures cells ascended gradually, and had two peaks on d18 and d48, during the period of culture. CONCLUSION: The cultured cells from S. japonicum schistosomula degenerated progressively.

Key words: Schistosoma japonicum, schistosomula, cultured cells, SOD, MDA