编码肝片吸虫GST和猪蛔虫GST基因的测序及同源性分析

中国寄生虫学与寄生虫病杂志 ›› 1998, Vol. 16 ›› Issue (4): 256-259.

编码肝片吸虫GST和猪蛔虫GST基因的测序及同源性分析

孙文宇; 刘述先

中国预防医学科学院寄生虫病研究所

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-08-28 发布日期:1998-08-28

SEQUENCING AND HOMOLOGY ANALYSIS OF cDNAS ENCODING FhGST AND AsGST *

Sun Wenyu; Liu Shuxian

Institute of Parasitic Diseases **; Chinese Academy of Preventive Medicine; Shanghai 200025

Received:1900-01-01 Revised:1900-01-01 Online:1998-08-28 Published:1998-08-28

摘要/Abstract

摘要： 目的：比较肝片吸虫GST（FhGST）、猪蛔虫GST（AsGST）基因与日本血吸虫26GST（Sj26GST）间的同源性。方法：以盐酸胍／氯化铯梯度超速离心法提取肝片吸虫和猪蛔虫的总RNA，合成两对特异性引物，通过反转录－PCR将所需的编码GST的cDNA扩增出来，并将PCR产物直接测序。应用DNASIS软件比较FhGST、AsGST基因与Sj26GST间的同源性。结果：测序得到FhGST403bp片段和AsGST411bp片段。结论：FhGST和AsGST片段与
Sj26GST的cD－NA在核苷酸水平上的同源性分别为59％和54％，两片段间的同源性为52％。

关键词: FhGST, AsGST, 反转录－PCR, 同源性

Abstract:

AIM: To study the homologies between FhGST, AsGST and Sj26GST. METHODS: Total RNAs
were extracted from Fasciola hepatica(Fh) and Ascaris suum(As) by guanidine hydrochloride/CsCl
ultra centrifugation. Two pairs of primers were designed according to FhGST and AsGST sequences.
RT PCR was carried out using total RNA as template. PCR products were directly sequenced. Then
the homology analysis was made using DNASIS software on nucleotide level. RESULTS: A FhGST403 bp
fragment and a AsGST411 bp fragment were obtained and they both located in the open reading frame. CONCLUSION: On nucleotide level, the homologies are 59% and 54% between the two cloned fragments and Schistosoma japonicum 26 kDa GST (Sj26GST) respectively

Key words: FhGST, AsGST, RT PCR, homology

孙文宇;刘述先. 编码肝片吸虫GST和猪蛔虫GST基因的测序及同源性分析[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(4): 256-259.

SunWenyu;LiuShuxian. SEQUENCING AND HOMOLOGY ANALYSIS OF cDNAS ENCODING FhGST AND AsGST *[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1998, 16(4): 256-259.

[1]	周瑞敏，张红卫*，邓艳，钱丹，刘颖，陈伟奇，颜秋叶，苏云普，赵旭东，许汴利. 2例输入性卵形疟的实验室检测[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(2): 10-127-130.
[2]	胡明洁1，方强2 *，吴守伟1，汤必奎1，张静1，焦玉萌2，夏惠2，沈继龙3. 间日疟原虫安徽地域株乳酸脱氢酶基因序列同源性分析[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(2): 20-148-150.
[3]	李雅杰;滕美君;伦永志;李达;张永轻. 蓝氏贾第鞭毛虫表面变异抗原基因的克隆与序列测定[J]. 中国寄生虫学与寄生虫病杂志, 2008, 26(3): 9-202.
[4]	刘殿武;李文玲;张丽梅. 猪囊尾蚴一种蛋白抗原基因的分子克隆[J]. 中国寄生虫学与寄生虫病杂志, 2004, 22(5): 14-305.
[5]	吕芳丽;胡孝素;敬保迁;马莹. 我国山丘疫区和平原疫区利什曼原虫分离株基因组DNA基因型分析[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(6): 432-435.
[6]	吕芳丽;胡孝素;汤海妹;曾凡亚;张义正;戴保民. 我国山丘疫区与平原疫区利什曼虫分离株分子核型分析[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(3): 181-184.
[7]	吕洪刚,胡孝素. 利什曼原虫动基体DNA微环同源性的分析及其克隆[J]. 中国寄生虫学与寄生虫病杂志, 1988, 6(S1): 38-39.