细粒棘球蚴生发细胞体外培养的实验观察

中国寄生虫学与寄生虫病杂志 ›› 1997, Vol. 15 ›› Issue (6): 392-394.

细粒棘球蚴生发细胞体外培养的实验观察

陆家海¹; 程维兴¹; 郭中敏²; 冯德元¹; 陈启军³; 李德昌³; 郭固²

1 兰州军区乌鲁木齐总医院; 2 新疆畜牧科学院; 3 解放军农牧大学

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-12-28 发布日期:1997-12-28

IN VITRO CULTIVATION OF THE GERMINAL CELLSFROM LARVAL ECHINOCOCCUS GRANULOSUS

Lu Jiahai¹; Cheng Weixing¹; Guo Zhongmin²; Feng Deyuan¹; Chen Qijun³; Li Dechang³; Guo Gu²

1 Urumqi General Hospital of Lanzhou Command; Urumqi 830000 2 Xinjiang Academy of Animal Science; Urumqi 830000 3 University of Agriculture and Anima Science, PLA, Chang chun 130062

Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

摘要/Abstract

摘要： 目的 :为培育人源细粒棘球蚴细胞系 (株 ) ,以供用于免疫预防研究。方法 :用外科方法从临床确诊的细粒棘球蚴患者的肝脏中取出包囊 ,以生发层和原头节为培养材料 ,采用RPMI1640、199和改良 DMEM培养液 ,用鼠尾胶原蛋白包被和不包被的培养瓶 ,对其进行初培养 ,并进行对比观察。结果 :以胶原蛋白作为支撑材料 ,应用改良 DMEM培养液培养的人源细粒棘球蚴生发层和原头节效果优于其他 ,已成功地培育了人源细粒棘球蚴细胞系 ,并传至第 2 0代。原代培养时间需 2 8d- 4 5d,3代以内细胞为多形态性。结论 :建立了适合人源细粒棘球蚴细胞体外培养的方法。

关键词: 人源细粒棘球蚴, 细胞培养, 细胞系

Abstract: AIM:To establish a cell line of larval Echinococcus granulosus. METHODS:The germinal cells and protoscoleces were obtained surgically from the patient with hydatidosis.The materials of germinal layer and protoscoleces were perpared for primary cultures. Trypsin was used for the enzymatic release of the monodispersed cells from the protoscoleces. The culture media were RPMI1640, 199 and improved DMEM with 15% - 20% fetal calf serum. RESULTS:Using improved DMEM medium and rat- tail collagen- coated flasks were suitable for cells on growth of larval Echinococcus granulosus, and was established cell line successfully. It has been 20 passages up to now. The times of primary cultures needed 28- 45 day. The cultured cells at passage 1- 3 were varying in size and shape. CONCLUSION: A method was established for culture cells of larval Echinococcus granulosus from patient.

Key words: Larval Echinococcus granulosus, in vitro cell culture, cell line

陆家海;程维兴;郭中敏;冯德元;陈启军;李德昌;郭固. 细粒棘球蚴生发细胞体外培养的实验观察[J]. 中国寄生虫学与寄生虫病杂志, 1997, 15(6): 392-394.

LuJiahai;ChengWeixing;GuoZhongmin;FengDeyuan;ChenQijun;LiDechang;GuoGu. IN VITRO CULTIVATION OF THE GERMINAL CELLSFROM LARVAL ECHINOCOCCUS GRANULOSUS[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1997, 15(6): 392-394.

[1]	李锴, 吴宏烨, 范俊杰, 王芬, 刘许诺, 张静, 叶彬. 鼠细粒棘球蚴囊壁消化残片的生发细胞培养试验[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(6): 571-577.
[2]	刘英杰1，徐静2，黄红莹1，许国强1. 旋毛虫排泄分泌蛋白对人肝癌HepG2细胞增殖的抑制作用[J]. 中国寄生虫学与寄生虫病杂志, 2015, 33(4): 20-315-317.
[3]	叶青;朱俊勇;钟沁萍;蒋明森;董惠芬. 湖北钉螺肝脏细胞原代培养的初步研究[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(6): 10-482.
[4]	林雪迟;曾庆仁;龚燕飞;言敢威;沈杰. 用组织化学方法鉴别日本血吸虫细胞来源的研究[J]. 中国寄生虫学与寄生虫病杂志, 2004, 22(4): 11-242.
[5]	彭延;蒋明森;钟沁萍;桂建芳;董惠芬. 湖北钉螺细胞原代培养的初步研究[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(3): 15-178.
[6]	黄克和;杨世广;唐建霞. 从感染小鼠获得微小隐孢子虫卵囊方法的改进[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(6): 12-362.
[7]	范虹;董惠芬;蒋明森;钟沁萍;明珍平. 日本血吸虫成虫培养液中氨基酸及葡萄糖含量的动态变化[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(1): 13-47.
[8]	陆家海;冯德元;郭中敏;李德昌;郭固. 人源细粒棘球蚴染色体G-和C-带初步分析[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(4): 17-249.
[9]	刘金明;顾惠明;王爱华;陈燕军. 细粒棘球蚴生发层细胞系的建立[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(5): 353-356.
[10]	刘杨;张永浩;李哲;姚向民. 蒿甲醚体外抗弓形虫作用的实验研究[J]. 中国寄生虫学与寄生虫病杂志, 1997, 15(6): 366-369.
[11]	蓝明扬,赵郁光,陆伟贤,吴昀,王尧. 两种按蚊细胞的游离氨基酸代谢的研究[J]. 中国寄生虫学与寄生虫病杂志, 1993, 11(4): 302-303.
[12]	庄国正,孟佩云,安克贵. 细胞培养和液氮冻存弓形虫的研究[J]. 中国寄生虫学与寄生虫病杂志, 1992, 10(2): 153-153.
[13]	刘玉珍,李得垣,谭德奎. 家猪卫氏并殖吸虫的染色体核型观察[J]. 中国寄生虫学与寄生虫病杂志, 1990, 8(2): 145-145.
[14]	王兴相,陈志强. 大鼠感染约氏疟原虫后的保护性免疫[J]. 中国寄生虫学与寄生虫病杂志, 1990, 8(2): 150-150.
[15]	周述龙. 日本血吸虫体外培养的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 1989, 7(1): 61-63.