中国寄生虫学与寄生虫病杂志 ›› 1997, Vol. 15 ›› Issue (6): 378-381.

• 论著 • 上一篇    下一篇

日本血吸虫 NADH脱氢酶 1和细胞色素 C氧化酶 1基因部分序列(英文)

邱持平1; 张立华2; E.Sorensen2; D.P.McManus2   

  1. 1 中国预防医学科学院寄生虫病研究所; 2 Molecular Helminthology Laboratory, Tropical Health Program, The Queensland Institute of Medical Research, Australia
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-12-28 发布日期:1997-12-28

PARTIAL SEQUENCE OF NADH DEHYDROGENASE 1 AND CYTOCHROME C OXIDASE 1 GENE OF SCHISTOSOMA JAPONICUM

Qiu Chiping1; Zhang Lihua2; E.Sorensen2; D.P.Mc Manus2   

  1. 1 Institute of Parasitic Diseases, Chinese Academy of Prevantive Medicine, Shanghai 200025 2 Molecular Helminthology Laboratory, Tropical Health Program, The Queensland Institute of Medical Research, Australia
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

摘要: 目的 :测定和比较日本血吸虫浙江株和安徽株 NADH脱氢酶 1( NADH1)和细胞色素 C氧化酶 1( Cytc1)基因部分序列。方法:用 PCR方法从特定的引物扩增出 NADH1和 Cytc1DNA基因 ,然后进行测序。结果 :获得两株日本血吸虫成虫 NADH1和 Cytc1基因及其序列。结论 :两株日本血吸虫成虫 NADH1基因及其部分序列除第 382位核苷酸不同 ( C/T)外 ,其余的均相同。两株日本血吸虫成虫Cytc1基因及其部分序列完全相同。

关键词: 日本血吸虫, NADH脱氢酶1, 细胞色素C氧化酶1, PCR, DNA序列

Abstract: AIM:To determine and compare NADH dehydrogenase1( NADH1) and cytochrome C oxidase1( Cytc1) partial
gene sequences of Zhejiang and Anhui isolates of Schistosoma japonicum. METHODS: NADH1 and Cytc1 genes were amplified by PCR using special primers corresponding with 44 1and 370 bp of the genomic DNAs, and subsequently sequenced. RESULTS:NADH1 and Cytc1 gene sequences were obtained from the two isolates. CONCLUSION: The partial sequences of the NADH1 genes were essentially identical for both isolates with only one nucleotide difference (C/T ) at position 382. The Cytc1 sequences were virtually identical for the two isolates.

Key words: Schistosom a japonicum, NADH dehydrogenase1, cytochrome C oxidase1, PCR, sequence