关键词: 核酸疫苗, 裂殖子表面蛋白1, 真核表达质粒, T细胞刺激位点, 聚合酶链式反应

Abstract: AIM:To construct hybrid nucleic acid vaccines which contain Plasmodium falciparum merozoite surface protein 1 (MSP1 ) block 17 gene and gene fragment coding for several T cell epitopes. METHODS:MSP1 block 17 gene of FUP strain was connected with a polyvalent gene fragment which contains several T cell epitopes from MSA1 ,MSA2 ,RESA,IL- 1 and Tetanus toxin, the connected gene fragment (hybrid gene,HG) was cloned into eukaryotic expression plasmid pCDNA3.1 (- ) ,VR1012 for intracellular expression,VR1012/TPA for secretion, the recombinants were identified by PCR and restriction enzyme digestion. RESULTS& CONCLUSION: The hybrid nucieic acid vaccines: VR 1012/HG, VR 1012/TPA/HG and pCDNA 3.1/HG were successfully constructed.

Key words: Nucleic acid vaccine, merozoite surface protein1, eukaryotic expression plasmid, T cell epitope, PCR