关键词: 日本血吸虫病, 循环表膜抗原, 单克隆抗体, 独特型/抗独特型抑制试验, 斑点-ELISA

Abstract: A monoclonal antibody (designated 8SE) against membrane antigen of adult Schistosomo japonicum labeled with HRP was used in dot-ELISA (direct method) to detect schistosomal circulating membrane antigen. Sera from 48 parasitologically confirmed schistosomiasis cases were tested, 39(81.396) were positive. No false positive reaction was found in sera from 24 healthy controls. No cross reaction was detected in sera from clonorchiasis or paragoni-miasis in 18 cases each. This results suggest that circulating membrane antigen does exist in patients with schistosomiasis and it might be used as a complementary method for diagnosis of schistosomiasis. A preliminary result of idiotype/antiidiotype interaction inhibition reaction for detecting circulating membrane antigen was also presented.