中国寄生虫学与寄生虫病杂志 ›› 1990, Vol. 8 ›› Issue (4): 284-287.

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感染食蟹猴疟原虫的大劣按蚊血淋巴和胃水解物氨基酸分析

黄复生,祁兆平,周成贵,孙玮   

  1. 第三军医大学寄生虫学教研室; 第三军医大学寄生虫学教研室; 第三军医大学仪器中心; 第三军医大学仪器中心
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1990-11-30 发布日期:2017-01-09

ANALYSIS OF AMINO ACIDS IN HEMOLYMPH AND ACID HYDROLYSATES OF MIDGUTS OF ANOPHELES DIRVS INFECTED WITH PLASMODIVM CYNOMOLGI

  • Received:2017-01-09 Revised:2017-01-09 Online:1990-11-30 Published:2017-01-09

摘要: 大劣按蚊感染食蟹猴疟原虫B株后第10d,血淋巴内的蛋氨酸、异亮氨酸、亮氨酸、鸟氨酸、赖氨酸含量均较对照组低。第9d蚊胃水解物分析结果表明,感染蚊胃中的多数氨基酸含量增加,其中缬氨酸、蛋氨酸、酪氨酸的含量是对照组3倍以上,氨基酸的总量比对照组高70%以上。

关键词: 食蟹猴疟原虫, 大劣按蚊, 血淋巴, 蚊胃水解物, 氨基酸

Abstract: Quantitative determinations of free amino acids in hemolymph and acid hydrolysates of midguts of female Anopheles dims infected with Plasmodium cynomolgi bastianellii were carried out and the results were compared with those of noninfected mosquitoes. On day 10 after infected blood meal, the contents of methionine, isoleucine, leucine, ornithine, lysine in the hemolymph of infected mosquitoes markedly decreased as compared with those in the controls. However, the quantitative analysis of the amino acids of the acid hydrolysates of the midguts from infected mosquitoes on day 9 after an infected blood meal showed that the content of their total amino acids was 70 % more than that in the controls, with special reference to aspartic acid, glutamic acid, valine, methionine, isoleucine, leucine, phenylalanine, tryptophan.