中国寄生虫学与寄生虫病杂志 ›› 1988, Vol. 6 ›› Issue (S1): 170-170.

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McAb-ELISA for detection of circulating antigen in schistosomiasis (I) Preparation, selection of monoclonal antibodies and preliminary test.

  

  • 收稿日期:2017-01-10 修回日期:2017-01-10 出版日期:1988-12-30 发布日期:2017-01-10

  • Received:2017-01-10 Revised:2017-01-10 Online:1988-12-30 Published:2017-01-10

摘要: Fusion tests between spleen cells from BALB/c mice mfected with Schistosoma japonicum or S-mansoni cercariae with Sp2/0 myeloma were carried out.More than 30 cell lines of monoclonal anti-bodies were obtained.Their target antigens were from tegument,gut and egg respectively.The prelimi-nary tests showed that the antibody against gut-associated polysaccharide antigen was of the highestactivity.This antibody can be usec in sandwich ELISA to detect trichloroacetic acid soluble antigen in