关键词: 中华按蚊, 比较研究, 蛋白质多肽, 二维电泳, 考马斯亮蓝G, 分子量, 等电点, 郑州, 银染色法, 凝胶

Abstract: A comparison of protein variation was made for the first time between Anopheles sinensis from Wuxi and Zhengzhou by two dimentional slab gel electrophoresis followed by sensitive silver staining method. A total of 142-144 proteins were detected, among Which, 35 were found to be variable. Two of the proteins showed only quantitative di-fference, whereas the rest 33 differed in both their molecular weights and isoelectric points. The variant proteins have been numbered. 16 of the 142 proteins in An. sinensis from Wuxi are variants (i.e. 11.3%), as compared to 19 of the 144 proteins in An. sinensis from Zhengzhou (i.e. 13.2%), the occurrence of variant proteins being 12.2% in total.