关键词: 循环免疫复合物, 特异性抗原, 酶解, 日本血吸虫, 免疫电泳, 双向对流, 晚期血吸虫病, 寄生虫学, 抗体水平, 人血清

Abstract: A new method of two-direction counter-immunoelectrophoresis (TD-CIEP and TD-ELACIEP) for detection of specific antigen and antibody of enzyme-dissociated schisto-some circulating immune complexes (SCIC) in infected rabbits and advanced schistosomia-sis cases was reported. Results showed that the detection rate was markedly increased in the enzyme-dissociated sera as compared with that of non-dissociated sera. The levels of antigen and antibodies were found to be correlated to the period and intensity of infection. The detected antigen was solely originated from the adult worm. Comparison of sensitivity of both methods showed that TD-ELACIEP was much more sensitive than TD-CIEP either for detection of antigen or antibodies. Of 54 sera of advanced schistosomiasis cases the detection rate of schistosome antigen or antibodies by TD-ELACIEP in enzyme-dissociated and non-dissociated serum samples was 50 and 25.9%, respectively. Only 9 cases( 16.7%) were COP positive with COP rate^5%. Sera from 25 healthy persons revealed no false positive reaction by either method. The present results indicated that TD-ELACIEP of dissociated SCIC may be helpful in immunological diagnosis of advanced schis-tasomiasis.