中国寄生虫学与寄生虫病杂志 ›› 1987, Vol. 5 ›› Issue (1): 22-24.

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对流免疫电泳检测粪内贾第虫抗原诊断贾第虫病

卢思奇,王正仪,张月清,温艳   

  1. 北京热带医学研究所
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1987-02-28 发布日期:2017-01-12

DIAGNOSING GIARDIASIS BY DETECTING GIARDIA LAMBLIA ANTIGEN IN FECAL MATTER WITH COUNTERIMMUNOELECTROPHOKESIS

  • Received:2017-01-12 Revised:2017-01-12 Online:1987-02-28 Published:2017-01-12

摘要: 对贾第虫患者的粪便用对流免疫电泳(CIE)法,测出其中存在贾第虫抗原。35例患者中有33例(94%)呈阳性,而40例正常人和64例其他急性腹泻患者(包括41例急性肠炎和23例急性细菌性痢疾)粪便的相应检测均为阴性。治疗后随着虫体的消失,CIE反应亦逐渐转阴。15只受染长爪沙鼠中有14只(93.3%),自接受包囊后第2日起至第25日止,粪便CIE均呈阳性。本法是诊断贾第虫现症感染和考核药物疗效的一个有效方法。

关键词: 贾第虫病, 对流免疫电泳, 抗原诊断, 急性细菌性痢疾, 滋养体, 抗血清, 热带医学, ELISA, 硫酸锌浮聚法, 十二指肠引流液

Abstract: Fecal specimens of 35 parasitologically confirmed cases of giardiasis, 41 acute, gastroenteritis, 23 acute baciUary dysentery, 40 normal persons and 15 jirds experimentally infected with G. lamblia were tested with conterimmunoelectrophoresis (CIE). It was found that 33(94%) out of the 35 giardiasis patients and 14(93%) out of 15 infected jirds showed positive reaction, while fecal specimens of other cases, normal persons, or normal jirds all showed negative reaction. Besides, CIE was performed in 4 giardiasis patients before and after metronidazole treatment. Prior to metronidazole, they were all CIE positive, one day post metronidazole, 3 of them were still CIE positive, and Giardia cysts or trophozoites were also present in their stools. However, from the second day onwards, all of them became CIE negative, while cysts or trophozoites also disappeared from their stools. Apparently, detecting Giardia antigen in fecal specimens with CIE of feces is not only a sensitive tool for detecting current infection, but also a, useful tool for evaluating therapeutic effects in giardiasis.