中国寄生虫学与寄生虫病杂志 ›› 1986, Vol. 4 ›› Issue (2): 86-89.
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李建新,周元昌,魏尧梅
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摘要: 采用体外培养方法,对恶性疟原虫FCC-1/HN株入侵人红细胞进行了观察,结果为:1.人血型糖蛋白A在50及100μg/ml时抑制入侵率分别达91与100%;卵类粘蛋白在100μg/ml时抑制40%。2.红细胞用唾液酸酶或胰蛋白酶处理后,疟原虫入侵率减少33~37%。3.D-葡糖胺HCI、唾液酸及N-乙酰-D-葡糖胺有较强的抑制入侵作用,但无协同作用.4.麦胚凝集素在100μg/ml时表现明显的抑制效应。
关键词: 血型糖蛋白A, 恶性疟原虫FCC-/HN株, 裂殖子, 唾液酸酶, 人红细胞膜, 凝集素, 原虫率, 葡糖胺, 卵类粘蛋白, 聚丙烯酞胺凝胶电泳
Abstract: The in vitro effect of different sugars and glcoproteins on the invasion of FCC-1/HN strain of P. falciparum merozoite into human erythrocyte is presented.1. Pure human glycophorin A inhibited invasion by 91% at 50μg/ml, and by 100% at 100μg/ml. Hen ovomucoid had 40% inhibitory effect at 100μg/ml.2. Human erythrocytes treated with sialidase or trypsin are less susceptible to merozoite invasion.3. D-glucosamine HC1, sialic and N-acetyl-D-glucosamine all had definitely inhibitory effect on merozoite invasion, however, no synergic effect was detected.4. Concanvalin A had no effect on merozoite invasion at 100μg/ml, while wheat germ agglutinin had a clearly inhibitory effect at the same concentration.
李建新,周元昌,魏尧梅. 人血型糖蛋白A及糖类对恶性疟原虫裂殖子入侵人红细胞的影响[J]. 中国寄生虫学与寄生虫病杂志, 1986, 4(2): 86-89.
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