关键词: 约氏疟原虫, 裂殖子, 裂殖体, 分离方法, 感染红细胞, 鸡红细胞, 红细胞感染率, 红细胞悬液, 蔗糖溶液, 感染小鼠

Abstract: Relatively pure preparations of schizonts and merozoites are required for studying the antigenicity of different stages of malaria parasite. Several methods of concentrating parasitized erythrocytes as well as isolating merozoites were used and compared. Eryth-rocytes parasitized with schizonts and large trophozoites can be obtained by overlaying the infected blood on 17.5% Ficoll solution and centrifuged. The interface layer contained schizonts mainly while the intermediate layer was rich in trophozoites. By using ConA-Sepharose 4B column, relatively pure and large amount of merozoites can be isolated, the purity of merozoites being more than 90%, with 5.4% large trophozoites and less than 1% red blood cells.