中国寄生虫学与寄生虫病杂志 ›› 1985, Vol. 3 ›› Issue (1): 35-37.
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黄先翔,韩家俊
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摘要： 本文报告711条美洲钩虫成虫在幼犬血清、199培养基、小牛血清、台氏液等七种培养基中的培养结果。其中以幼犬血清和含少量199培养基的幼犬血清培养效果为好,虫体在体外存活半月以上者占64.2％,一月以上者占21％,雌虫最长存活52天,雄虫43天,并有虫体交配,在培养基中查见了受精卵、未受精卵和钩虫幼虫。本实验体外培养美洲钩虫成虫成功,为研究美洲钩虫各个发育阶段创造了一定条件,为探索人体两种钩虫的差异提供了一条途径。
关键词: 美洲钩虫, 体外培养, 未受精卵, 十二指肠钩虫, 幼犬, 血清培养, 感染期幼虫, 人工感染, 存活时间, 快速测试
Abstract: This paper reports the results of in vitro cultivation of adult Necator americanus in different media such as puppy serum, calf serum, medium 199 and Tyrode's solution. Among them, puppy serum medium and that containing a little amount of medium 1999 gave better results. 64.2% of worms survived for two weeks and 21% of them over one month in these two media. The longest survival time of female and male worms was 52 and 43 days respectively.Copulation was observed in culture medium and fertilized, and unfertilized eggs and larvae were also found.The success of in vitro culture og N. americanus has provided a new approach to study the developing stages of N. americanus and the difference between N. americanus, and Ancylostoma duodenale.
黄先翔,韩家俊. 体外培养美洲钩虫成虫的研究[J]. 中国寄生虫学与寄生虫病杂志, 1985, 3(1): 35-37.
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